Effect of calcium antagonists, calcium channel blockers and calmodulin inhibitors on the growth and encystation of Entamoeba histolytica and E. invadens

A. Makioka, M. Kumagai, H. Ohtomo, S. Kobayashi, T. Takeuchi

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Abstract

The effects of calcium antagonists, calcium channel blockers, and calmodulin inhibitors on the growth of Entamoeba histolytica and the growth and encystation of Entamoeba invadens were examined. Calcium chelators ethyleneglycol bis (β-aminoethyl ether)-N,N'-tetraacetate (EGTA) and ethylene-diaminetetraacetate (EDTA) inhibited the growth of both Entamoeba and also the encystation of E. invadens in a dose-dependent manner, with EDTA being more effective than EGTA. A putative antagonist of intracellular calcium flux, 8-(N,N-diethylamino) octyl-3,4,5-trimethoxybenzoate (TMB-8) also inhibited both growth and encystation, with the E. histolytica being more sensitive than E. invadens, and with the growth of E. invadens being more sensitive than encystation. The slow Na+-Ca2+ channel blockers bepridil and verapamil inhibited both growth and encystation. Bepridil was more effective than verapamil. The calmodulin (CaM) inhibitors, W-7 (N-(6-aminohexyl)-chloro-1-naphtalene sulphonamide) and trifluoperazine (TFP), were also inhibitory for both the growth and encystation; TFP was more effective than W-7, and encystation was more sensitive than growth in E. invadens. These results indicate that extracellular calcium ions, amebic intracellular calcium flux, calcium channels, and a CaM-dependent process contribute to the growth and encystation of Entamoeba.

Original languageEnglish
Pages (from-to)833-837
Number of pages5
JournalParasitology Research
Volume87
Issue number10
DOIs
Publication statusPublished - 2001

Fingerprint

Entamoeba histolytica
calcium channel blockers
encystment
Growth Inhibitors
Calcium Channel Blockers
calmodulin
Calmodulin
antagonists
Calcium
calcium
Entamoeba
Growth
Bepridil
trifluoperazine
Trifluoperazine
verapamil
Egtazic Acid
Verapamil
ethylene
calcium channels

ASJC Scopus subject areas

  • Parasitology

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Effect of calcium antagonists, calcium channel blockers and calmodulin inhibitors on the growth and encystation of Entamoeba histolytica and E. invadens. / Makioka, A.; Kumagai, M.; Ohtomo, H.; Kobayashi, S.; Takeuchi, T.

In: Parasitology Research, Vol. 87, No. 10, 2001, p. 833-837.

Research output: Contribution to journalArticle

Makioka, A. ; Kumagai, M. ; Ohtomo, H. ; Kobayashi, S. ; Takeuchi, T. / Effect of calcium antagonists, calcium channel blockers and calmodulin inhibitors on the growth and encystation of Entamoeba histolytica and E. invadens. In: Parasitology Research. 2001 ; Vol. 87, No. 10. pp. 833-837.
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