Abstract
This study investigated the effect of chronic digitalis glycoside use on β-adrenergic sympathetic activities in heart failure. Twenty-two Japanese white rabbits were anesthetized by intravenous injection of chloral hydrate. Aortic regurgitation (AR) was produced by perforating aortic valves in 14 rabbits. Digoxin was given for 1 week to 7 rabbits with AR (AR + Dig) and saline to 7 rabbits with AR (AR + C). Sham operation was performed in the remaining 8 rabbits (S). The left ventricular end-diastolic pressure was higher in AR + C than S (p < 0.05). It was lower in AR + Dig than AR + C (p < 0.05). Cardiac output was lower in AR + C than S (p < 0.05). There was no difference between AR + Dig and S. Both the left ventricular end-diastolic and end-systolic diameters were larger in AR + C (p < 0.05) than S, but they were similar between AR + Dig and S. Plasma norepinephrine level was lower in AR + Dig than AR + C. Myocardial β-adrenergic receptors number determined by radioligand binding assay using 30-800 pM 125I-iodocyanopindolol was lower in AR + C than S (28.8 ± 7.9 vs. 69.9 ± 12.3 fmol/mg protein, p < 0.05). It was higher in AR + Dig (39.9 ± 9.8) than AR + C (p < 0.05). Myocardial norepinephrine content was lower in both AR + C (p < 0.05) and AR + Dig than S (p < 0.05). Thus, digitalis glycosides exert favorable effects on β-adrenergic sympathetic activities in addition to the effects on hemodynamic variables in this animal model of heart failure.
| Original language | English |
|---|---|
| Pages (from-to) | 263-272 |
| Number of pages | 10 |
| Journal | Japanese Heart Journal |
| Volume | 38 |
| Issue number | 2 |
| Publication status | Published - 1997 |
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Keywords
- β-adrenergic receptor
- Aortic regurgitation
- Digitalis glycosides
- Heart failure
- Rabbits
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Effect of chronic digoxin on β-adrenergic receptors in rabbits with heart failure. / Albajinai, Wurin; Yoshikawa, Tsutomu; Suzuki, Masahiro; Nagami, Keiichi; Wainai, Yumiko; Handa, Shunnosuke.
In: Japanese Heart Journal, Vol. 38, No. 2, 1997, p. 263-272.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of chronic digoxin on β-adrenergic receptors in rabbits with heart failure
AU - Albajinai, Wurin
AU - Yoshikawa, Tsutomu
AU - Suzuki, Masahiro
AU - Nagami, Keiichi
AU - Wainai, Yumiko
AU - Handa, Shunnosuke
PY - 1997
Y1 - 1997
N2 - This study investigated the effect of chronic digitalis glycoside use on β-adrenergic sympathetic activities in heart failure. Twenty-two Japanese white rabbits were anesthetized by intravenous injection of chloral hydrate. Aortic regurgitation (AR) was produced by perforating aortic valves in 14 rabbits. Digoxin was given for 1 week to 7 rabbits with AR (AR + Dig) and saline to 7 rabbits with AR (AR + C). Sham operation was performed in the remaining 8 rabbits (S). The left ventricular end-diastolic pressure was higher in AR + C than S (p < 0.05). It was lower in AR + Dig than AR + C (p < 0.05). Cardiac output was lower in AR + C than S (p < 0.05). There was no difference between AR + Dig and S. Both the left ventricular end-diastolic and end-systolic diameters were larger in AR + C (p < 0.05) than S, but they were similar between AR + Dig and S. Plasma norepinephrine level was lower in AR + Dig than AR + C. Myocardial β-adrenergic receptors number determined by radioligand binding assay using 30-800 pM 125I-iodocyanopindolol was lower in AR + C than S (28.8 ± 7.9 vs. 69.9 ± 12.3 fmol/mg protein, p < 0.05). It was higher in AR + Dig (39.9 ± 9.8) than AR + C (p < 0.05). Myocardial norepinephrine content was lower in both AR + C (p < 0.05) and AR + Dig than S (p < 0.05). Thus, digitalis glycosides exert favorable effects on β-adrenergic sympathetic activities in addition to the effects on hemodynamic variables in this animal model of heart failure.
AB - This study investigated the effect of chronic digitalis glycoside use on β-adrenergic sympathetic activities in heart failure. Twenty-two Japanese white rabbits were anesthetized by intravenous injection of chloral hydrate. Aortic regurgitation (AR) was produced by perforating aortic valves in 14 rabbits. Digoxin was given for 1 week to 7 rabbits with AR (AR + Dig) and saline to 7 rabbits with AR (AR + C). Sham operation was performed in the remaining 8 rabbits (S). The left ventricular end-diastolic pressure was higher in AR + C than S (p < 0.05). It was lower in AR + Dig than AR + C (p < 0.05). Cardiac output was lower in AR + C than S (p < 0.05). There was no difference between AR + Dig and S. Both the left ventricular end-diastolic and end-systolic diameters were larger in AR + C (p < 0.05) than S, but they were similar between AR + Dig and S. Plasma norepinephrine level was lower in AR + Dig than AR + C. Myocardial β-adrenergic receptors number determined by radioligand binding assay using 30-800 pM 125I-iodocyanopindolol was lower in AR + C than S (28.8 ± 7.9 vs. 69.9 ± 12.3 fmol/mg protein, p < 0.05). It was higher in AR + Dig (39.9 ± 9.8) than AR + C (p < 0.05). Myocardial norepinephrine content was lower in both AR + C (p < 0.05) and AR + Dig than S (p < 0.05). Thus, digitalis glycosides exert favorable effects on β-adrenergic sympathetic activities in addition to the effects on hemodynamic variables in this animal model of heart failure.
KW - β-adrenergic receptor
KW - Aortic regurgitation
KW - Digitalis glycosides
KW - Heart failure
KW - Rabbits
UR - http://www.scopus.com/inward/record.url?scp=0030990179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030990179&partnerID=8YFLogxK
M3 - Article
C2 - 9201113
AN - SCOPUS:0030990179
VL - 38
SP - 263
EP - 272
JO - International Heart Journal
JF - International Heart Journal
SN - 1349-2365
IS - 2
ER -