Effect of chronic digoxin on β-adrenergic receptors in rabbits with heart failure

Wurin Albajinai, Tsutomu Yoshikawa, Masahiro Suzuki, Keiichi Nagami, Yumiko Wainai, Shunnosuke Handa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study investigated the effect of chronic digitalis glycoside use on β-adrenergic sympathetic activities in heart failure. Twenty-two Japanese white rabbits were anesthetized by intravenous injection of chloral hydrate. Aortic regurgitation (AR) was produced by perforating aortic valves in 14 rabbits. Digoxin was given for 1 week to 7 rabbits with AR (AR + Dig) and saline to 7 rabbits with AR (AR + C). Sham operation was performed in the remaining 8 rabbits (S). The left ventricular end-diastolic pressure was higher in AR + C than S (p < 0.05). It was lower in AR + Dig than AR + C (p < 0.05). Cardiac output was lower in AR + C than S (p < 0.05). There was no difference between AR + Dig and S. Both the left ventricular end-diastolic and end-systolic diameters were larger in AR + C (p < 0.05) than S, but they were similar between AR + Dig and S. Plasma norepinephrine level was lower in AR + Dig than AR + C. Myocardial β-adrenergic receptors number determined by radioligand binding assay using 30-800 pM 125I-iodocyanopindolol was lower in AR + C than S (28.8 ± 7.9 vs. 69.9 ± 12.3 fmol/mg protein, p < 0.05). It was higher in AR + Dig (39.9 ± 9.8) than AR + C (p < 0.05). Myocardial norepinephrine content was lower in both AR + C (p < 0.05) and AR + Dig than S (p < 0.05). Thus, digitalis glycosides exert favorable effects on β-adrenergic sympathetic activities in addition to the effects on hemodynamic variables in this animal model of heart failure.

Original languageEnglish
Pages (from-to)263-272
Number of pages10
JournalJapanese Heart Journal
Volume38
Issue number2
Publication statusPublished - 1997

Fingerprint

Aortic Valve Insufficiency
Digoxin
Adrenergic Receptors
Heart Failure
Rabbits
Digitalis Glycosides
Adrenergic Agents
Norepinephrine
Iodocyanopindolol
Chloral Hydrate
Radioligand Assay

Keywords

  • β-adrenergic receptor
  • Aortic regurgitation
  • Digitalis glycosides
  • Heart failure
  • Rabbits

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Albajinai, W., Yoshikawa, T., Suzuki, M., Nagami, K., Wainai, Y., & Handa, S. (1997). Effect of chronic digoxin on β-adrenergic receptors in rabbits with heart failure. Japanese Heart Journal, 38(2), 263-272.

Effect of chronic digoxin on β-adrenergic receptors in rabbits with heart failure. / Albajinai, Wurin; Yoshikawa, Tsutomu; Suzuki, Masahiro; Nagami, Keiichi; Wainai, Yumiko; Handa, Shunnosuke.

In: Japanese Heart Journal, Vol. 38, No. 2, 1997, p. 263-272.

Research output: Contribution to journalArticle

Albajinai, W, Yoshikawa, T, Suzuki, M, Nagami, K, Wainai, Y & Handa, S 1997, 'Effect of chronic digoxin on β-adrenergic receptors in rabbits with heart failure', Japanese Heart Journal, vol. 38, no. 2, pp. 263-272.
Albajinai W, Yoshikawa T, Suzuki M, Nagami K, Wainai Y, Handa S. Effect of chronic digoxin on β-adrenergic receptors in rabbits with heart failure. Japanese Heart Journal. 1997;38(2):263-272.
Albajinai, Wurin ; Yoshikawa, Tsutomu ; Suzuki, Masahiro ; Nagami, Keiichi ; Wainai, Yumiko ; Handa, Shunnosuke. / Effect of chronic digoxin on β-adrenergic receptors in rabbits with heart failure. In: Japanese Heart Journal. 1997 ; Vol. 38, No. 2. pp. 263-272.
@article{8fb771b1356e4c22b43d101e936dab44,
title = "Effect of chronic digoxin on β-adrenergic receptors in rabbits with heart failure",
abstract = "This study investigated the effect of chronic digitalis glycoside use on β-adrenergic sympathetic activities in heart failure. Twenty-two Japanese white rabbits were anesthetized by intravenous injection of chloral hydrate. Aortic regurgitation (AR) was produced by perforating aortic valves in 14 rabbits. Digoxin was given for 1 week to 7 rabbits with AR (AR + Dig) and saline to 7 rabbits with AR (AR + C). Sham operation was performed in the remaining 8 rabbits (S). The left ventricular end-diastolic pressure was higher in AR + C than S (p < 0.05). It was lower in AR + Dig than AR + C (p < 0.05). Cardiac output was lower in AR + C than S (p < 0.05). There was no difference between AR + Dig and S. Both the left ventricular end-diastolic and end-systolic diameters were larger in AR + C (p < 0.05) than S, but they were similar between AR + Dig and S. Plasma norepinephrine level was lower in AR + Dig than AR + C. Myocardial β-adrenergic receptors number determined by radioligand binding assay using 30-800 pM 125I-iodocyanopindolol was lower in AR + C than S (28.8 ± 7.9 vs. 69.9 ± 12.3 fmol/mg protein, p < 0.05). It was higher in AR + Dig (39.9 ± 9.8) than AR + C (p < 0.05). Myocardial norepinephrine content was lower in both AR + C (p < 0.05) and AR + Dig than S (p < 0.05). Thus, digitalis glycosides exert favorable effects on β-adrenergic sympathetic activities in addition to the effects on hemodynamic variables in this animal model of heart failure.",
keywords = "β-adrenergic receptor, Aortic regurgitation, Digitalis glycosides, Heart failure, Rabbits",
author = "Wurin Albajinai and Tsutomu Yoshikawa and Masahiro Suzuki and Keiichi Nagami and Yumiko Wainai and Shunnosuke Handa",
year = "1997",
language = "English",
volume = "38",
pages = "263--272",
journal = "International Heart Journal",
issn = "1349-2365",
publisher = "International Heart Journal Association",
number = "2",

}

TY - JOUR

T1 - Effect of chronic digoxin on β-adrenergic receptors in rabbits with heart failure

AU - Albajinai, Wurin

AU - Yoshikawa, Tsutomu

AU - Suzuki, Masahiro

AU - Nagami, Keiichi

AU - Wainai, Yumiko

AU - Handa, Shunnosuke

PY - 1997

Y1 - 1997

N2 - This study investigated the effect of chronic digitalis glycoside use on β-adrenergic sympathetic activities in heart failure. Twenty-two Japanese white rabbits were anesthetized by intravenous injection of chloral hydrate. Aortic regurgitation (AR) was produced by perforating aortic valves in 14 rabbits. Digoxin was given for 1 week to 7 rabbits with AR (AR + Dig) and saline to 7 rabbits with AR (AR + C). Sham operation was performed in the remaining 8 rabbits (S). The left ventricular end-diastolic pressure was higher in AR + C than S (p < 0.05). It was lower in AR + Dig than AR + C (p < 0.05). Cardiac output was lower in AR + C than S (p < 0.05). There was no difference between AR + Dig and S. Both the left ventricular end-diastolic and end-systolic diameters were larger in AR + C (p < 0.05) than S, but they were similar between AR + Dig and S. Plasma norepinephrine level was lower in AR + Dig than AR + C. Myocardial β-adrenergic receptors number determined by radioligand binding assay using 30-800 pM 125I-iodocyanopindolol was lower in AR + C than S (28.8 ± 7.9 vs. 69.9 ± 12.3 fmol/mg protein, p < 0.05). It was higher in AR + Dig (39.9 ± 9.8) than AR + C (p < 0.05). Myocardial norepinephrine content was lower in both AR + C (p < 0.05) and AR + Dig than S (p < 0.05). Thus, digitalis glycosides exert favorable effects on β-adrenergic sympathetic activities in addition to the effects on hemodynamic variables in this animal model of heart failure.

AB - This study investigated the effect of chronic digitalis glycoside use on β-adrenergic sympathetic activities in heart failure. Twenty-two Japanese white rabbits were anesthetized by intravenous injection of chloral hydrate. Aortic regurgitation (AR) was produced by perforating aortic valves in 14 rabbits. Digoxin was given for 1 week to 7 rabbits with AR (AR + Dig) and saline to 7 rabbits with AR (AR + C). Sham operation was performed in the remaining 8 rabbits (S). The left ventricular end-diastolic pressure was higher in AR + C than S (p < 0.05). It was lower in AR + Dig than AR + C (p < 0.05). Cardiac output was lower in AR + C than S (p < 0.05). There was no difference between AR + Dig and S. Both the left ventricular end-diastolic and end-systolic diameters were larger in AR + C (p < 0.05) than S, but they were similar between AR + Dig and S. Plasma norepinephrine level was lower in AR + Dig than AR + C. Myocardial β-adrenergic receptors number determined by radioligand binding assay using 30-800 pM 125I-iodocyanopindolol was lower in AR + C than S (28.8 ± 7.9 vs. 69.9 ± 12.3 fmol/mg protein, p < 0.05). It was higher in AR + Dig (39.9 ± 9.8) than AR + C (p < 0.05). Myocardial norepinephrine content was lower in both AR + C (p < 0.05) and AR + Dig than S (p < 0.05). Thus, digitalis glycosides exert favorable effects on β-adrenergic sympathetic activities in addition to the effects on hemodynamic variables in this animal model of heart failure.

KW - β-adrenergic receptor

KW - Aortic regurgitation

KW - Digitalis glycosides

KW - Heart failure

KW - Rabbits

UR - http://www.scopus.com/inward/record.url?scp=0030990179&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030990179&partnerID=8YFLogxK

M3 - Article

VL - 38

SP - 263

EP - 272

JO - International Heart Journal

JF - International Heart Journal

SN - 1349-2365

IS - 2

ER -