Abstract
The effect of colchicine on mouse synovioblasts (B-cells) was investigated by electron microscopy and electron microscopic morphometry with special consideration to intracellular movement and secretion of secretory granules. The volume density of microtubules decreased by one-fifth to one-tenth for 1-36 hr after colchicine administration, and that of secretory granules increased twofold for 3-24 hr. Secretory granules were accumulated around the Golgi apparatus for 3-6 hr, and in the cell periphery for 12-24 hr. Small vesicles positive for periodic acid-thiocarbohydrazide-silver proteinate stain were recognized beneath the plasma membrane for 12-24 hr. These results appear to indicate that intracellular translocation of secretory granules toward the plasma membrane is mediated by the microtubular system, and that most of their contents are released into the extracellular space via small vesicles.
| Original language | English |
|---|---|
| Pages (from-to) | 160-167 |
| Number of pages | 8 |
| Journal | Journal of Electron Microscopy |
| Volume | 33 |
| Issue number | 2 |
| Publication status | Published - 1984 |
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Effect of colchicine on mouse synovioblasts (B-cells). / Okada, Y.; Nakanishi, I.
In: Journal of Electron Microscopy, Vol. 33, No. 2, 1984, p. 160-167.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of colchicine on mouse synovioblasts (B-cells)
AU - Okada, Y.
AU - Nakanishi, I.
PY - 1984
Y1 - 1984
N2 - The effect of colchicine on mouse synovioblasts (B-cells) was investigated by electron microscopy and electron microscopic morphometry with special consideration to intracellular movement and secretion of secretory granules. The volume density of microtubules decreased by one-fifth to one-tenth for 1-36 hr after colchicine administration, and that of secretory granules increased twofold for 3-24 hr. Secretory granules were accumulated around the Golgi apparatus for 3-6 hr, and in the cell periphery for 12-24 hr. Small vesicles positive for periodic acid-thiocarbohydrazide-silver proteinate stain were recognized beneath the plasma membrane for 12-24 hr. These results appear to indicate that intracellular translocation of secretory granules toward the plasma membrane is mediated by the microtubular system, and that most of their contents are released into the extracellular space via small vesicles.
AB - The effect of colchicine on mouse synovioblasts (B-cells) was investigated by electron microscopy and electron microscopic morphometry with special consideration to intracellular movement and secretion of secretory granules. The volume density of microtubules decreased by one-fifth to one-tenth for 1-36 hr after colchicine administration, and that of secretory granules increased twofold for 3-24 hr. Secretory granules were accumulated around the Golgi apparatus for 3-6 hr, and in the cell periphery for 12-24 hr. Small vesicles positive for periodic acid-thiocarbohydrazide-silver proteinate stain were recognized beneath the plasma membrane for 12-24 hr. These results appear to indicate that intracellular translocation of secretory granules toward the plasma membrane is mediated by the microtubular system, and that most of their contents are released into the extracellular space via small vesicles.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021751422&partnerID=8YFLogxK
M3 - Article
VL - 33
SP - 160
EP - 167
JO - Microscopy (Oxford, England)
JF - Microscopy (Oxford, England)
SN - 2050-5698
IS - 2
ER -