Effect of colchicine on mouse synovioblasts (B-cells)

Y. Okada, I. Nakanishi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The effect of colchicine on mouse synovioblasts (B-cells) was investigated by electron microscopy and electron microscopic morphometry with special consideration to intracellular movement and secretion of secretory granules. The volume density of microtubules decreased by one-fifth to one-tenth for 1-36 hr after colchicine administration, and that of secretory granules increased twofold for 3-24 hr. Secretory granules were accumulated around the Golgi apparatus for 3-6 hr, and in the cell periphery for 12-24 hr. Small vesicles positive for periodic acid-thiocarbohydrazide-silver proteinate stain were recognized beneath the plasma membrane for 12-24 hr. These results appear to indicate that intracellular translocation of secretory granules toward the plasma membrane is mediated by the microtubular system, and that most of their contents are released into the extracellular space via small vesicles.

Original languageEnglish
Pages (from-to)160-167
Number of pages8
JournalJournal of Electron Microscopy
Volume33
Issue number2
Publication statusPublished - 1984

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colchicine
Secretory Vesicles
Colchicine
Cell membranes
mice
B-Lymphocytes
Cells
membranes
secretions
Electron microscopy
electron microscopy
Silver
silver
Cell Membrane
acids
Acids
Electrons
Extracellular Space
Golgi Apparatus
cells

ASJC Scopus subject areas

  • Instrumentation

Cite this

Effect of colchicine on mouse synovioblasts (B-cells). / Okada, Y.; Nakanishi, I.

In: Journal of Electron Microscopy, Vol. 33, No. 2, 1984, p. 160-167.

Research output: Contribution to journalArticle

Okada, Y & Nakanishi, I 1984, 'Effect of colchicine on mouse synovioblasts (B-cells)', Journal of Electron Microscopy, vol. 33, no. 2, pp. 160-167.
Okada, Y. ; Nakanishi, I. / Effect of colchicine on mouse synovioblasts (B-cells). In: Journal of Electron Microscopy. 1984 ; Vol. 33, No. 2. pp. 160-167.
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