Abstract
Articular cartilage has a poor ability to regenerate and repair itself. To restore cartilage defects, a method has been developed by culturing autologous chondrocytes to create a three dimensional tissue and then implanting the cultured tissue. However, articular chondrocytes easily leads to de-differentiation state and loses their ability to synthesize the functional cartilaginous matrixes during in vitro culture. Therefore, it is important to maintain their differentiated phenotype during the expansion culture of chondrocytes. The objective of this study is to develop a novel culturing methodology combined surface modification and mechanical stimuli on articular cartilage. To develop a surface-modified substrate, a SUS316L stainless plate was treated by Fine Particle Peening (FPP) technique using alumina particles. The chondrocytes were cultured on the modified stainless plate and stimulated by fluid-induced shear stress using a custom-made flow culture system. As a result, proliferation rate was increased and phenotypic-change of chondrocytes was also observed by culturing the chondrocytes on physically modified surface combined with fluid-induced shear stress. In addition, the synergetic effect on proliferation rate was observed by applying the surface modification and flow shear stress simultaneously.
| Original language | English |
|---|---|
| Pages (from-to) | 324-329 |
| Number of pages | 6 |
| Journal | Nippon Kinzoku Gakkaishi/Journal of the Japan Institute of Metals |
| Volume | 79 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2015 Jun 1 |
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Keywords
- Cell differentiation
- Cell proliferation
- Chondrocyte
- Fine particle peening
- Shear stress
ASJC Scopus subject areas
- Mechanics of Materials
- Materials Chemistry
- Metals and Alloys
- Condensed Matter Physics
Cite this
Effect of complex stimuli of shear stress and surface modification on proliferation and phenotype of chondrocyte. / Nakai, Soichiro; Miyata, Shogo; Komotori, Jun.
In: Nippon Kinzoku Gakkaishi/Journal of the Japan Institute of Metals, Vol. 79, No. 6, 01.06.2015, p. 324-329.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of complex stimuli of shear stress and surface modification on proliferation and phenotype of chondrocyte
AU - Nakai, Soichiro
AU - Miyata, Shogo
AU - Komotori, Jun
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Articular cartilage has a poor ability to regenerate and repair itself. To restore cartilage defects, a method has been developed by culturing autologous chondrocytes to create a three dimensional tissue and then implanting the cultured tissue. However, articular chondrocytes easily leads to de-differentiation state and loses their ability to synthesize the functional cartilaginous matrixes during in vitro culture. Therefore, it is important to maintain their differentiated phenotype during the expansion culture of chondrocytes. The objective of this study is to develop a novel culturing methodology combined surface modification and mechanical stimuli on articular cartilage. To develop a surface-modified substrate, a SUS316L stainless plate was treated by Fine Particle Peening (FPP) technique using alumina particles. The chondrocytes were cultured on the modified stainless plate and stimulated by fluid-induced shear stress using a custom-made flow culture system. As a result, proliferation rate was increased and phenotypic-change of chondrocytes was also observed by culturing the chondrocytes on physically modified surface combined with fluid-induced shear stress. In addition, the synergetic effect on proliferation rate was observed by applying the surface modification and flow shear stress simultaneously.
AB - Articular cartilage has a poor ability to regenerate and repair itself. To restore cartilage defects, a method has been developed by culturing autologous chondrocytes to create a three dimensional tissue and then implanting the cultured tissue. However, articular chondrocytes easily leads to de-differentiation state and loses their ability to synthesize the functional cartilaginous matrixes during in vitro culture. Therefore, it is important to maintain their differentiated phenotype during the expansion culture of chondrocytes. The objective of this study is to develop a novel culturing methodology combined surface modification and mechanical stimuli on articular cartilage. To develop a surface-modified substrate, a SUS316L stainless plate was treated by Fine Particle Peening (FPP) technique using alumina particles. The chondrocytes were cultured on the modified stainless plate and stimulated by fluid-induced shear stress using a custom-made flow culture system. As a result, proliferation rate was increased and phenotypic-change of chondrocytes was also observed by culturing the chondrocytes on physically modified surface combined with fluid-induced shear stress. In addition, the synergetic effect on proliferation rate was observed by applying the surface modification and flow shear stress simultaneously.
KW - Cell differentiation
KW - Cell proliferation
KW - Chondrocyte
KW - Fine particle peening
KW - Shear stress
UR - http://www.scopus.com/inward/record.url?scp=84938823877&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938823877&partnerID=8YFLogxK
U2 - 10.2320/jinstmet.J2015009
DO - 10.2320/jinstmet.J2015009
M3 - Article
AN - SCOPUS:84938823877
VL - 79
SP - 324
EP - 329
JO - Nippon Kinzoku Gakkaishi/Journal of the Japan Institute of Metals
JF - Nippon Kinzoku Gakkaishi/Journal of the Japan Institute of Metals
SN - 0021-4876
IS - 6
ER -