Effect of cooling stimulus on collection efficiency of calf chondrocytes cultivated on metal surface

Yuta Kurashina, Shogo Miyata, Jun Komotori

Research output: Contribution to journalArticle

Abstract

A cell culture module capable of cooling stimulus to collect cells efficiently on a metal culture substrate was developed. We evaluated the cell collection ratio and morphology of the collected cells. Following a cooling stimulus (0 C) for 20 min, the number of collected cells was increased by 50% compared to that collected after trypsin treatment without pipetting from the metal culture substrate. Following the cooling stimulus, cells were observed by fluorescence microscopy and scanning electron microscopy; the cell filopodia were shrunken compared to non-cooling-stimulated cells. Furthermore, the combination of collagenase and cooling stimulation resulted in the collection of a comparable number of cells as that obtained using only trypsin. Thus, cell proliferation was improved compared to that following trypsin treatment. Therefore, this method can be applied for culturing cells that are susceptible to trypsin damage.

Original languageEnglish
Article numberijate001100060925
Pages (from-to)925-931
Number of pages7
JournalInternational Journal of Automation Technology
Volume11
Issue number6
DOIs
Publication statusPublished - 2017 Nov 1

Fingerprint

Cooling
Metals
Fluorescence microscopy
Cell proliferation
Substrates
Cell culture
Scanning electron microscopy

Keywords

  • Cell adherence
  • Cell proliferation
  • Chondrocyte
  • Fine particle peening
  • Surface modification

ASJC Scopus subject areas

  • Mechanical Engineering
  • Industrial and Manufacturing Engineering

Cite this

Effect of cooling stimulus on collection efficiency of calf chondrocytes cultivated on metal surface. / Kurashina, Yuta; Miyata, Shogo; Komotori, Jun.

In: International Journal of Automation Technology, Vol. 11, No. 6, ijate001100060925, 01.11.2017, p. 925-931.

Research output: Contribution to journalArticle

@article{57f7aaccad1c41f986bf7868c06d95db,
title = "Effect of cooling stimulus on collection efficiency of calf chondrocytes cultivated on metal surface",
abstract = "A cell culture module capable of cooling stimulus to collect cells efficiently on a metal culture substrate was developed. We evaluated the cell collection ratio and morphology of the collected cells. Following a cooling stimulus (0◦ C) for 20 min, the number of collected cells was increased by 50{\%} compared to that collected after trypsin treatment without pipetting from the metal culture substrate. Following the cooling stimulus, cells were observed by fluorescence microscopy and scanning electron microscopy; the cell filopodia were shrunken compared to non-cooling-stimulated cells. Furthermore, the combination of collagenase and cooling stimulation resulted in the collection of a comparable number of cells as that obtained using only trypsin. Thus, cell proliferation was improved compared to that following trypsin treatment. Therefore, this method can be applied for culturing cells that are susceptible to trypsin damage.",
keywords = "Cell adherence, Cell proliferation, Chondrocyte, Fine particle peening, Surface modification",
author = "Yuta Kurashina and Shogo Miyata and Jun Komotori",
year = "2017",
month = "11",
day = "1",
doi = "10.20965/ijat.2017.p0925",
language = "English",
volume = "11",
pages = "925--931",
journal = "International Journal of Automation Technology",
issn = "1881-7629",
publisher = "Fuji Technology Press",
number = "6",

}

TY - JOUR

T1 - Effect of cooling stimulus on collection efficiency of calf chondrocytes cultivated on metal surface

AU - Kurashina, Yuta

AU - Miyata, Shogo

AU - Komotori, Jun

PY - 2017/11/1

Y1 - 2017/11/1

N2 - A cell culture module capable of cooling stimulus to collect cells efficiently on a metal culture substrate was developed. We evaluated the cell collection ratio and morphology of the collected cells. Following a cooling stimulus (0◦ C) for 20 min, the number of collected cells was increased by 50% compared to that collected after trypsin treatment without pipetting from the metal culture substrate. Following the cooling stimulus, cells were observed by fluorescence microscopy and scanning electron microscopy; the cell filopodia were shrunken compared to non-cooling-stimulated cells. Furthermore, the combination of collagenase and cooling stimulation resulted in the collection of a comparable number of cells as that obtained using only trypsin. Thus, cell proliferation was improved compared to that following trypsin treatment. Therefore, this method can be applied for culturing cells that are susceptible to trypsin damage.

AB - A cell culture module capable of cooling stimulus to collect cells efficiently on a metal culture substrate was developed. We evaluated the cell collection ratio and morphology of the collected cells. Following a cooling stimulus (0◦ C) for 20 min, the number of collected cells was increased by 50% compared to that collected after trypsin treatment without pipetting from the metal culture substrate. Following the cooling stimulus, cells were observed by fluorescence microscopy and scanning electron microscopy; the cell filopodia were shrunken compared to non-cooling-stimulated cells. Furthermore, the combination of collagenase and cooling stimulation resulted in the collection of a comparable number of cells as that obtained using only trypsin. Thus, cell proliferation was improved compared to that following trypsin treatment. Therefore, this method can be applied for culturing cells that are susceptible to trypsin damage.

KW - Cell adherence

KW - Cell proliferation

KW - Chondrocyte

KW - Fine particle peening

KW - Surface modification

UR - http://www.scopus.com/inward/record.url?scp=85035071519&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85035071519&partnerID=8YFLogxK

U2 - 10.20965/ijat.2017.p0925

DO - 10.20965/ijat.2017.p0925

M3 - Article

VL - 11

SP - 925

EP - 931

JO - International Journal of Automation Technology

JF - International Journal of Automation Technology

SN - 1881-7629

IS - 6

M1 - ijate001100060925

ER -