Effect of cyclic compressive stimuli on mechanical anisotropy of chondrocyte-seeded agarose gel culture

Yuya Okuda, Rei Konishi, Shogo Miyata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Recently tissue engineering therapy culturing autologous chondrocytes in vitro to create three dimensional tissue for the replacement of damaged tissue has been developed. Although the tissue engineering is a useful approach to reconstruct cartilage in vitro, the mechanical property of the engineered cartilage is not sufficient to replace natural cartilage. Within the natural cartilage, cells and collagen fibers, which are main components of the cartilage, align in response to the direction of cyclic deformations induced by daily walking or other activities. Due to this collagen fiber alignment, the cartilage has mechanical anisotropy. In this study, we focus on the beneficial effect of dynamic compressive loading on tissue regeneration for articular cartilage. The purpose of this study is to create mechanically anisotropic cartilage by compressive stimuli during the culture.

Original languageEnglish
Pages (from-to)1736-1743
Number of pages8
JournalNihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C
Volume79
Issue number801
DOIs
Publication statusPublished - 2013

Fingerprint

Cartilage
Anisotropy
Gels
Tissue engineering
Collagen
Tissue
Tissue regeneration
Fibers
Mechanical properties

Keywords

  • Anisotropy
  • Bone and cartilage
  • Orthopaedic biomechanics
  • Regenerative medicine
  • Tissue engineering

ASJC Scopus subject areas

  • Mechanical Engineering
  • Mechanics of Materials
  • Industrial and Manufacturing Engineering

Cite this

@article{28e6d93f2b9f42a597dedddeb2770863,
title = "Effect of cyclic compressive stimuli on mechanical anisotropy of chondrocyte-seeded agarose gel culture",
abstract = "Recently tissue engineering therapy culturing autologous chondrocytes in vitro to create three dimensional tissue for the replacement of damaged tissue has been developed. Although the tissue engineering is a useful approach to reconstruct cartilage in vitro, the mechanical property of the engineered cartilage is not sufficient to replace natural cartilage. Within the natural cartilage, cells and collagen fibers, which are main components of the cartilage, align in response to the direction of cyclic deformations induced by daily walking or other activities. Due to this collagen fiber alignment, the cartilage has mechanical anisotropy. In this study, we focus on the beneficial effect of dynamic compressive loading on tissue regeneration for articular cartilage. The purpose of this study is to create mechanically anisotropic cartilage by compressive stimuli during the culture.",
keywords = "Anisotropy, Bone and cartilage, Orthopaedic biomechanics, Regenerative medicine, Tissue engineering",
author = "Yuya Okuda and Rei Konishi and Shogo Miyata",
year = "2013",
doi = "10.1299/kikaic.79.1736",
language = "English",
volume = "79",
pages = "1736--1743",
journal = "Nihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C",
issn = "0387-5024",
publisher = "Japan Society of Mechanical Engineers",
number = "801",

}

TY - JOUR

T1 - Effect of cyclic compressive stimuli on mechanical anisotropy of chondrocyte-seeded agarose gel culture

AU - Okuda, Yuya

AU - Konishi, Rei

AU - Miyata, Shogo

PY - 2013

Y1 - 2013

N2 - Recently tissue engineering therapy culturing autologous chondrocytes in vitro to create three dimensional tissue for the replacement of damaged tissue has been developed. Although the tissue engineering is a useful approach to reconstruct cartilage in vitro, the mechanical property of the engineered cartilage is not sufficient to replace natural cartilage. Within the natural cartilage, cells and collagen fibers, which are main components of the cartilage, align in response to the direction of cyclic deformations induced by daily walking or other activities. Due to this collagen fiber alignment, the cartilage has mechanical anisotropy. In this study, we focus on the beneficial effect of dynamic compressive loading on tissue regeneration for articular cartilage. The purpose of this study is to create mechanically anisotropic cartilage by compressive stimuli during the culture.

AB - Recently tissue engineering therapy culturing autologous chondrocytes in vitro to create three dimensional tissue for the replacement of damaged tissue has been developed. Although the tissue engineering is a useful approach to reconstruct cartilage in vitro, the mechanical property of the engineered cartilage is not sufficient to replace natural cartilage. Within the natural cartilage, cells and collagen fibers, which are main components of the cartilage, align in response to the direction of cyclic deformations induced by daily walking or other activities. Due to this collagen fiber alignment, the cartilage has mechanical anisotropy. In this study, we focus on the beneficial effect of dynamic compressive loading on tissue regeneration for articular cartilage. The purpose of this study is to create mechanically anisotropic cartilage by compressive stimuli during the culture.

KW - Anisotropy

KW - Bone and cartilage

KW - Orthopaedic biomechanics

KW - Regenerative medicine

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=84879995140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879995140&partnerID=8YFLogxK

U2 - 10.1299/kikaic.79.1736

DO - 10.1299/kikaic.79.1736

M3 - Article

VL - 79

SP - 1736

EP - 1743

JO - Nihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C

JF - Nihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C

SN - 0387-5024

IS - 801

ER -