Effect of cyclic stretch on tissue maturation in myoblast-laden hydrogel fibers

Shinako Bansai, Takashi Morikura, Hiroaki Onoe, Shogo Miyata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Engineering of the skeletal muscles has attracted attention for the restoration of damaged muscles from myopathy, injury, and extraction of malignant tumors. Reconstructing a three-dimensional muscle using living cells could be a promising approach. However, the regenerated tissue exhibits a weak construction force due to the insufficient tissue maturation. The purpose of this study is to establish the reconstruction system for the skeletal muscle. We used a cell-laden core-shell hydrogel microfiber as a three-dimensional culture to control the cellular orientation. Moreover, to mature the muscle tissue in the microfiber, we also developed a custom-made culture device for imposing cyclic stretch stimulation using a motorized stage and the fiber-grab system. As a result, the directions of the myotubes were oriented and the mature myotubes could be formed by cyclic stretch stimulation.

Original languageEnglish
Article number399
JournalMicromachines
Volume10
Issue number6
DOIs
Publication statusPublished - 2019 Jun 1

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Keywords

  • Core-shell hydrogel fiber
  • Cyclic stretch
  • Engineered muscle
  • Myoblast
  • Skeletal muscle

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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