Effect of dietary anti-urease immunoglobulin Y on Helicobacter pylori infection in Mongolian gerbils

Sachiko Nomura, Hidekazu Suzuki, Tatsuhiro Masaoka, Kumiko Kurabayashi, Hiromasa Ishii, Masaki Kitajima, Kikuo Nomoto, Toshifumi Hibi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Background and aim. Helicobacter pylori is known to be a major pathogenic factor in the development of gastritis, peptic ulcer disease and gastric cancer. Recently, chicken egg yolk immunoglobulin Y (IgY) has been recognized as an inexpensive antibody source for passive immunization against gastrointestinal infections. The present study was designed to investigate the effect of anti-urease IgY on H. pylori infection in Mongolian gerbils. Methods. H. pylori-infected Mongolian gerbils were administered a diet containing anti-urease IgY, with or without famotidine (F). After 10 weeks, bacterial culture and measurement of the gastric mucosal myeloperoxidase (MPO) activity were performed. In a second experiment, another group of gerbils was started on a diet containing F + IgY a week prior to H. pylori inoculation. After 9 weeks, these animals were examined. Results. In the H. pylori-infected gerbils, there were no significant differences in the level of H. pylori colonization among the different dietary and control groups. However, the MPO activity was significantly decreased in the H. pylori group administered the F + IgY diet compared with that in the H. pylori group administered the IgY, F, or control diet. Furthermore, in the gerbils administered the F + IgY diet prior to the bacterial inoculation, inhibition of H. pylori colonization and suppression of the elevated gastric mucosal MPO activity were observed. Conclusions. Oral administration of urease-specific IgY not only inhibited H. pylori disease activity in H. pylori-infected gerbils, but also prevented H. pylori colonization in those not yet infected. These encouraging results may pave the way for a novel therapeutic and prophylactic approach in the management of H. pylori-associated gastroduodenal disease.

Original languageEnglish
Pages (from-to)43-52
Number of pages10
JournalHelicobacter
Volume10
Issue number1
DOIs
Publication statusPublished - 2005

Fingerprint

Urease
Gerbillinae
Helicobacter Infections
Helicobacter pylori
Diet
Peroxidase
IgY
Stomach
Famotidine
Egg Yolk
Passive Immunization
Gastritis
Peptic Ulcer
Stomach Neoplasms
Oral Administration
Chickens

Keywords

  • Antacid
  • Egg yolk immunoglobulin
  • Helicobacter pylori
  • Mongolian gerbil
  • Oral passive immunization

ASJC Scopus subject areas

  • Gastroenterology
  • Microbiology

Cite this

Effect of dietary anti-urease immunoglobulin Y on Helicobacter pylori infection in Mongolian gerbils. / Nomura, Sachiko; Suzuki, Hidekazu; Masaoka, Tatsuhiro; Kurabayashi, Kumiko; Ishii, Hiromasa; Kitajima, Masaki; Nomoto, Kikuo; Hibi, Toshifumi.

In: Helicobacter, Vol. 10, No. 1, 2005, p. 43-52.

Research output: Contribution to journalArticle

Nomura, S, Suzuki, H, Masaoka, T, Kurabayashi, K, Ishii, H, Kitajima, M, Nomoto, K & Hibi, T 2005, 'Effect of dietary anti-urease immunoglobulin Y on Helicobacter pylori infection in Mongolian gerbils', Helicobacter, vol. 10, no. 1, pp. 43-52. https://doi.org/10.1111/j.1523-5378.2005.00290.x
Nomura, Sachiko ; Suzuki, Hidekazu ; Masaoka, Tatsuhiro ; Kurabayashi, Kumiko ; Ishii, Hiromasa ; Kitajima, Masaki ; Nomoto, Kikuo ; Hibi, Toshifumi. / Effect of dietary anti-urease immunoglobulin Y on Helicobacter pylori infection in Mongolian gerbils. In: Helicobacter. 2005 ; Vol. 10, No. 1. pp. 43-52.
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AU - Nomura, Sachiko

AU - Suzuki, Hidekazu

AU - Masaoka, Tatsuhiro

AU - Kurabayashi, Kumiko

AU - Ishii, Hiromasa

AU - Kitajima, Masaki

AU - Nomoto, Kikuo

AU - Hibi, Toshifumi

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N2 - Background and aim. Helicobacter pylori is known to be a major pathogenic factor in the development of gastritis, peptic ulcer disease and gastric cancer. Recently, chicken egg yolk immunoglobulin Y (IgY) has been recognized as an inexpensive antibody source for passive immunization against gastrointestinal infections. The present study was designed to investigate the effect of anti-urease IgY on H. pylori infection in Mongolian gerbils. Methods. H. pylori-infected Mongolian gerbils were administered a diet containing anti-urease IgY, with or without famotidine (F). After 10 weeks, bacterial culture and measurement of the gastric mucosal myeloperoxidase (MPO) activity were performed. In a second experiment, another group of gerbils was started on a diet containing F + IgY a week prior to H. pylori inoculation. After 9 weeks, these animals were examined. Results. In the H. pylori-infected gerbils, there were no significant differences in the level of H. pylori colonization among the different dietary and control groups. However, the MPO activity was significantly decreased in the H. pylori group administered the F + IgY diet compared with that in the H. pylori group administered the IgY, F, or control diet. Furthermore, in the gerbils administered the F + IgY diet prior to the bacterial inoculation, inhibition of H. pylori colonization and suppression of the elevated gastric mucosal MPO activity were observed. Conclusions. Oral administration of urease-specific IgY not only inhibited H. pylori disease activity in H. pylori-infected gerbils, but also prevented H. pylori colonization in those not yet infected. These encouraging results may pave the way for a novel therapeutic and prophylactic approach in the management of H. pylori-associated gastroduodenal disease.

AB - Background and aim. Helicobacter pylori is known to be a major pathogenic factor in the development of gastritis, peptic ulcer disease and gastric cancer. Recently, chicken egg yolk immunoglobulin Y (IgY) has been recognized as an inexpensive antibody source for passive immunization against gastrointestinal infections. The present study was designed to investigate the effect of anti-urease IgY on H. pylori infection in Mongolian gerbils. Methods. H. pylori-infected Mongolian gerbils were administered a diet containing anti-urease IgY, with or without famotidine (F). After 10 weeks, bacterial culture and measurement of the gastric mucosal myeloperoxidase (MPO) activity were performed. In a second experiment, another group of gerbils was started on a diet containing F + IgY a week prior to H. pylori inoculation. After 9 weeks, these animals were examined. Results. In the H. pylori-infected gerbils, there were no significant differences in the level of H. pylori colonization among the different dietary and control groups. However, the MPO activity was significantly decreased in the H. pylori group administered the F + IgY diet compared with that in the H. pylori group administered the IgY, F, or control diet. Furthermore, in the gerbils administered the F + IgY diet prior to the bacterial inoculation, inhibition of H. pylori colonization and suppression of the elevated gastric mucosal MPO activity were observed. Conclusions. Oral administration of urease-specific IgY not only inhibited H. pylori disease activity in H. pylori-infected gerbils, but also prevented H. pylori colonization in those not yet infected. These encouraging results may pave the way for a novel therapeutic and prophylactic approach in the management of H. pylori-associated gastroduodenal disease.

KW - Antacid

KW - Egg yolk immunoglobulin

KW - Helicobacter pylori

KW - Mongolian gerbil

KW - Oral passive immunization

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