Effect of dinitroaniline herbicides on the growth of Entamoeba histolytica

Asao Makioka, Masahiro Kumagai, Hiroshi Ohtomo, Seiki Kobayashi, Tsutomu Takeuchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effect of the dinitroaniline herbicides oryzalin and trifluralin on the growth of Entamoeba histolytica was examined. Oryzalin inhibited the growth of E. histolytica strain HM-1:IMSS. Trifluralin was less effective than oryzalin for this parasite. Entamoeba histolytica was more resistant to these dinitroanilines than other parasitic protozoa examined so far, including Leishmania spp., Trypanosoma brucei, Plasmodium falciparum, Toxoplasma gondii, and Cryptosporidium parvum. Colchicine, a potent microtubule inhibitor of animal cells, was much less effective for E. histolytica, even at very high concentrations. A reptilian parasite, Entamoeba invadens strain IP-1, examined for comparison, was more resistant to these dinitroanilines than E. histolytica. Accumulation of E. histolytica trophozoites in mitosis was observed after culture in 100 μM oryzalin. The inhibitory effect of oryzalin on the growth of E. histolytica trophozoites was abrogated by removal of the drug after exposure to 100 μM for 2 days. In parallel to the recovery of growth after removal of the drug, the percentage of trophozoites in mitosis was reduced to a normal level. The results indicate that treatment of trophozoites with oryzalin arrests mitosis and that its effect is reversible. Therefore, oryzalin is a useful tool for studies relating to the cell cycle of this parasite.

Original languageEnglish
Pages (from-to)607-610
Number of pages4
JournalJournal of Parasitology
Volume86
Issue number3
Publication statusPublished - 2000 Jun

Fingerprint

dinitroaniline herbicides
oryzalin
Entamoeba histolytica
Herbicides
herbicide
Trophozoites
trophozoites
trifluralin
parasite
Growth
Trifluralin
Mitosis
drug
mitosis
Parasites
parasites
inhibitor
Entamoeba
Cryptosporidium parvum
drugs

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Makioka, A., Kumagai, M., Ohtomo, H., Kobayashi, S., & Takeuchi, T. (2000). Effect of dinitroaniline herbicides on the growth of Entamoeba histolytica. Journal of Parasitology, 86(3), 607-610.

Effect of dinitroaniline herbicides on the growth of Entamoeba histolytica. / Makioka, Asao; Kumagai, Masahiro; Ohtomo, Hiroshi; Kobayashi, Seiki; Takeuchi, Tsutomu.

In: Journal of Parasitology, Vol. 86, No. 3, 06.2000, p. 607-610.

Research output: Contribution to journalArticle

Makioka, A, Kumagai, M, Ohtomo, H, Kobayashi, S & Takeuchi, T 2000, 'Effect of dinitroaniline herbicides on the growth of Entamoeba histolytica', Journal of Parasitology, vol. 86, no. 3, pp. 607-610.
Makioka A, Kumagai M, Ohtomo H, Kobayashi S, Takeuchi T. Effect of dinitroaniline herbicides on the growth of Entamoeba histolytica. Journal of Parasitology. 2000 Jun;86(3):607-610.
Makioka, Asao ; Kumagai, Masahiro ; Ohtomo, Hiroshi ; Kobayashi, Seiki ; Takeuchi, Tsutomu. / Effect of dinitroaniline herbicides on the growth of Entamoeba histolytica. In: Journal of Parasitology. 2000 ; Vol. 86, No. 3. pp. 607-610.
@article{82e0fb8bcc644e5dbf7e5b8592194ad4,
title = "Effect of dinitroaniline herbicides on the growth of Entamoeba histolytica",
abstract = "The effect of the dinitroaniline herbicides oryzalin and trifluralin on the growth of Entamoeba histolytica was examined. Oryzalin inhibited the growth of E. histolytica strain HM-1:IMSS. Trifluralin was less effective than oryzalin for this parasite. Entamoeba histolytica was more resistant to these dinitroanilines than other parasitic protozoa examined so far, including Leishmania spp., Trypanosoma brucei, Plasmodium falciparum, Toxoplasma gondii, and Cryptosporidium parvum. Colchicine, a potent microtubule inhibitor of animal cells, was much less effective for E. histolytica, even at very high concentrations. A reptilian parasite, Entamoeba invadens strain IP-1, examined for comparison, was more resistant to these dinitroanilines than E. histolytica. Accumulation of E. histolytica trophozoites in mitosis was observed after culture in 100 μM oryzalin. The inhibitory effect of oryzalin on the growth of E. histolytica trophozoites was abrogated by removal of the drug after exposure to 100 μM for 2 days. In parallel to the recovery of growth after removal of the drug, the percentage of trophozoites in mitosis was reduced to a normal level. The results indicate that treatment of trophozoites with oryzalin arrests mitosis and that its effect is reversible. Therefore, oryzalin is a useful tool for studies relating to the cell cycle of this parasite.",
author = "Asao Makioka and Masahiro Kumagai and Hiroshi Ohtomo and Seiki Kobayashi and Tsutomu Takeuchi",
year = "2000",
month = "6",
language = "English",
volume = "86",
pages = "607--610",
journal = "Journal of Parasitology",
issn = "0022-3395",
publisher = "American Society of Parasitologists",
number = "3",

}

TY - JOUR

T1 - Effect of dinitroaniline herbicides on the growth of Entamoeba histolytica

AU - Makioka, Asao

AU - Kumagai, Masahiro

AU - Ohtomo, Hiroshi

AU - Kobayashi, Seiki

AU - Takeuchi, Tsutomu

PY - 2000/6

Y1 - 2000/6

N2 - The effect of the dinitroaniline herbicides oryzalin and trifluralin on the growth of Entamoeba histolytica was examined. Oryzalin inhibited the growth of E. histolytica strain HM-1:IMSS. Trifluralin was less effective than oryzalin for this parasite. Entamoeba histolytica was more resistant to these dinitroanilines than other parasitic protozoa examined so far, including Leishmania spp., Trypanosoma brucei, Plasmodium falciparum, Toxoplasma gondii, and Cryptosporidium parvum. Colchicine, a potent microtubule inhibitor of animal cells, was much less effective for E. histolytica, even at very high concentrations. A reptilian parasite, Entamoeba invadens strain IP-1, examined for comparison, was more resistant to these dinitroanilines than E. histolytica. Accumulation of E. histolytica trophozoites in mitosis was observed after culture in 100 μM oryzalin. The inhibitory effect of oryzalin on the growth of E. histolytica trophozoites was abrogated by removal of the drug after exposure to 100 μM for 2 days. In parallel to the recovery of growth after removal of the drug, the percentage of trophozoites in mitosis was reduced to a normal level. The results indicate that treatment of trophozoites with oryzalin arrests mitosis and that its effect is reversible. Therefore, oryzalin is a useful tool for studies relating to the cell cycle of this parasite.

AB - The effect of the dinitroaniline herbicides oryzalin and trifluralin on the growth of Entamoeba histolytica was examined. Oryzalin inhibited the growth of E. histolytica strain HM-1:IMSS. Trifluralin was less effective than oryzalin for this parasite. Entamoeba histolytica was more resistant to these dinitroanilines than other parasitic protozoa examined so far, including Leishmania spp., Trypanosoma brucei, Plasmodium falciparum, Toxoplasma gondii, and Cryptosporidium parvum. Colchicine, a potent microtubule inhibitor of animal cells, was much less effective for E. histolytica, even at very high concentrations. A reptilian parasite, Entamoeba invadens strain IP-1, examined for comparison, was more resistant to these dinitroanilines than E. histolytica. Accumulation of E. histolytica trophozoites in mitosis was observed after culture in 100 μM oryzalin. The inhibitory effect of oryzalin on the growth of E. histolytica trophozoites was abrogated by removal of the drug after exposure to 100 μM for 2 days. In parallel to the recovery of growth after removal of the drug, the percentage of trophozoites in mitosis was reduced to a normal level. The results indicate that treatment of trophozoites with oryzalin arrests mitosis and that its effect is reversible. Therefore, oryzalin is a useful tool for studies relating to the cell cycle of this parasite.

UR - http://www.scopus.com/inward/record.url?scp=0033917205&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033917205&partnerID=8YFLogxK

M3 - Article

C2 - 10864261

AN - SCOPUS:0033917205

VL - 86

SP - 607

EP - 610

JO - Journal of Parasitology

JF - Journal of Parasitology

SN - 0022-3395

IS - 3

ER -