Effect of dithiothreitol on angiotensin II receptor type II in rat ovarian cultured granulosa cells

Junji Ohnishi, Masami Tanaka, Mitsuhide Naruse, Satoshi Usuki, Kazuo Murakami, Hitoshi Miyazaki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Dithiothreitol markedly increased the ligand binding affinity of angiotensin II (AII) receptor type II (AT2) without affecting its antagonist selectivity in cultured ovarian granulosa cells, demonstrating that this AT2 is of the dithiothreitol-sensitive type. Dithiothreitol is useful for specifically detecting low levels of the AT2 in the ovary, where it plays roles that are probably related to atresia.

Original languageEnglish
Pages (from-to)286-288
Number of pages3
JournalBBA - Biomembranes
Volume1192
Issue number2
DOIs
Publication statusPublished - 1994 Jun 22

Keywords

  • Angiotensin II
  • Antagonist
  • Granulosa cell
  • Receptor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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