Effect of Estradiol and Ethynylestradiol on Microtubule Distribution in Chinese Hamster V79 Cells

Yoshihiro Sato; Yumiko Sakakibara; Taiko Oda; Eriko Aizu-Yokota; Ken Ichinoseki

doi:10.1248/cpb.40.182

Effect of Estradiol and Ethynylestradiol on Microtubule Distribution in Chinese Hamster V79 Cells

Yoshihiro Sato, Yumiko Sakakibara, Taiko Oda, Eriko Aizu-Yokota, Ken Ichinoseki

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

The effects of estradiol (E₂) and ethynylestradiol (EE₂) on the chromosome number and cellular microtubule architecture of Chinese hamster V79 cells were studied using fluorescent anti-tubulin antibody. Treatment with 20 pM E₂ for 48 h induced only a small amount of tetraploid cells, but the normal microtubule network was disrupted completely by only 3h of treatment. This data reveals that E₂ has higher microtubule-disruptive activity than diethylstilbestrol in V79 cells.

Original language	English
Pages (from-to)	182-184
Number of pages	3
Journal	Chemical and Pharmaceutical Bulletin
Volume	40
Issue number	1
DOIs	https://doi.org/10.1248/cpb.40.182
Publication status	Published - 1992

Keywords

aneuploidy
chromosome aberration
estradiol
ethynylestradiol
immunofluorescence
microtubule distribution

ASJC Scopus subject areas

Chemistry(all)
Drug Discovery

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10.1248/cpb.40.182

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abstract = "The effects of estradiol (E2) and ethynylestradiol (EE2) on the chromosome number and cellular microtubule architecture of Chinese hamster V79 cells were studied using fluorescent anti-tubulin antibody. Treatment with 20 pM E2 for 48 h induced only a small amount of tetraploid cells, but the normal microtubule network was disrupted completely by only 3h of treatment. This data reveals that E2 has higher microtubule-disruptive activity than diethylstilbestrol in V79 cells.",

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AU - Sato, Yoshihiro

AU - Sakakibara, Yumiko

AU - Oda, Taiko

AU - Aizu-Yokota, Eriko

AU - Ichinoseki, Ken

PY - 1992

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AB - The effects of estradiol (E2) and ethynylestradiol (EE2) on the chromosome number and cellular microtubule architecture of Chinese hamster V79 cells were studied using fluorescent anti-tubulin antibody. Treatment with 20 pM E2 for 48 h induced only a small amount of tetraploid cells, but the normal microtubule network was disrupted completely by only 3h of treatment. This data reveals that E2 has higher microtubule-disruptive activity than diethylstilbestrol in V79 cells.

KW - aneuploidy

KW - chromosome aberration

KW - estradiol

KW - ethynylestradiol

KW - immunofluorescence

KW - microtubule distribution

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Effect of Estradiol and Ethynylestradiol on Microtubule Distribution in Chinese Hamster V79 Cells

Abstract

Keywords

ASJC Scopus subject areas

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