Effect of ethanol on mouse hepatitis virus-induced cytotoxicity

Hidetsugu Saito, Tetsuya Masuda, Akira Tashita, Yoshiro Ishiwata, Shoji Yokochi, Takahiko Mitani, Hiromasa Ishii

Research output: Contribution to journalArticle

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Abstract

The effect of ethanol on cells infected with mouse hepatitis virus (MHV) was investigated. After MHV infection of competent cells, NCTC1469, ethanol was added to the culture at various concentrations, and the viability of cells was measured using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide. To examine the possible involvement of the ethanol metabolite, acetaldehyde, alcohol dehydrogenase activity was measured in NCTC1469 cells. Ethanol alone did not show cytotoxicity against NCTC1469 cells at concentrations from 0.125% to 2%. After infection with MHV, the viability of cells decreased, and this decrease was further enhanced, dose-dependently, by the addition of ethanol. The activity of alcohol dehydrogenase in the cells was below the detectable level. The same phenomena were also demonstrated in cells infected with influenza virus and Herpes simplex virus. These results demonstrate that ethanol enhances MHV-mediated cytotoxicity; this exacerbation of cytotoxicity by ethanol is suggested to be an effect common to cytopathic virus-infected cells.

Original languageEnglish
Pages (from-to)679-683
Number of pages5
JournalJournal of Gastroenterology
Volume31
Issue number5
DOIs
Publication statusPublished - 1996

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Murine hepatitis virus
Ethanol
Alcohol Dehydrogenase
Microbial Viability
Virus Diseases
Simplexvirus
Orthomyxoviridae
Bromides
Cell Survival
Viruses

Keywords

  • Ethanol
  • Mitochondrial function
  • Mouse hepatitis virus
  • Virus-infected cell

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Saito, H., Masuda, T., Tashita, A., Ishiwata, Y., Yokochi, S., Mitani, T., & Ishii, H. (1996). Effect of ethanol on mouse hepatitis virus-induced cytotoxicity. Journal of Gastroenterology, 31(5), 679-683. https://doi.org/10.1007/BF02347616

Effect of ethanol on mouse hepatitis virus-induced cytotoxicity. / Saito, Hidetsugu; Masuda, Tetsuya; Tashita, Akira; Ishiwata, Yoshiro; Yokochi, Shoji; Mitani, Takahiko; Ishii, Hiromasa.

In: Journal of Gastroenterology, Vol. 31, No. 5, 1996, p. 679-683.

Research output: Contribution to journalArticle

Saito, H, Masuda, T, Tashita, A, Ishiwata, Y, Yokochi, S, Mitani, T & Ishii, H 1996, 'Effect of ethanol on mouse hepatitis virus-induced cytotoxicity', Journal of Gastroenterology, vol. 31, no. 5, pp. 679-683. https://doi.org/10.1007/BF02347616
Saito, Hidetsugu ; Masuda, Tetsuya ; Tashita, Akira ; Ishiwata, Yoshiro ; Yokochi, Shoji ; Mitani, Takahiko ; Ishii, Hiromasa. / Effect of ethanol on mouse hepatitis virus-induced cytotoxicity. In: Journal of Gastroenterology. 1996 ; Vol. 31, No. 5. pp. 679-683.
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