Effect of external pH on the cytoplasmic and vacuolar phs in mung bean root-tip cells: A 31P nuclear magnetic resonance study

Keiichi Torimitsu, Yoshiaki Yazaki, Kinuyo Nagasuka, Eiji Ohta, Makoto Sakata

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The effect of the external pH on the intracellular pH in mung bean (Vigna mungo (L.) Hepper) root-tip cells was investigated with the 31P nuclear magnetic resonance (NMR) method. The 31P NMR spectra showed three peaks caused by cytoplasmic G-6-P, cytoplasmic Pi and vacuolar Pi. The cytoplasmic and vacuolar pHs could be determined by comparing the Pi chemical shifts with the titration curve. When the external pH was changed over a range from pH 3 to 10, the cytoplasmic pH showed smaller changes than the vacuolar pH, suggesting that the former is regulated more strictly than the latter. The H+-ATPase inhibitor, DCCD, caused the breakdown of the mechanism that regulates the intracellular pH. H+-ATPase appears to have an important part in the regulation of the intracellular pH.

Original languageEnglish
Pages (from-to)1403-1409
Number of pages7
JournalPlant and Cell Physiology
Issue number8
Publication statusPublished - 1984 Dec



  • 31P NMR
  • Intracellular pH
  • Root tip cells
  • Vigna mungo

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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