Effect of external pH on the cytoplasmic and vacuolar phs in mung bean root-tip cells

A 31P nuclear magnetic resonance study

Keiichi Torimitsu, Yoshiaki Yazaki, Kinuyo Nagasuka, Eiji Ohta, Makoto Sakata

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The effect of the external pH on the intracellular pH in mung bean (Vigna mungo (L.) Hepper) root-tip cells was investigated with the 31P nuclear magnetic resonance (NMR) method. The 31P NMR spectra showed three peaks caused by cytoplasmic G-6-P, cytoplasmic Pi and vacuolar Pi. The cytoplasmic and vacuolar pHs could be determined by comparing the Pi chemical shifts with the titration curve. When the external pH was changed over a range from pH 3 to 10, the cytoplasmic pH showed smaller changes than the vacuolar pH, suggesting that the former is regulated more strictly than the latter. The H+-ATPase inhibitor, DCCD, caused the breakdown of the mechanism that regulates the intracellular pH. H+-ATPase appears to have an important part in the regulation of the intracellular pH.

Original languageEnglish
Pages (from-to)1403-1409
Number of pages7
JournalPlant and Cell Physiology
Volume25
Issue number8
Publication statusPublished - 1984 Dec

Fingerprint

Mung Bean
Meristem
Nuclear Magnetic Resonance
ATP Synthase
mung beans
Pi
root tips
nuclear magnetic resonance
nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Roots
Cell
Chemical shift
Titration
Inhibitor
Breakdown
inhibitor
Strictly
cells

Keywords

  • 31P NMR
  • Intracellular pH
  • Root tip cells
  • Vigna mungo

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Effect of external pH on the cytoplasmic and vacuolar phs in mung bean root-tip cells : A 31P nuclear magnetic resonance study. / Torimitsu, Keiichi; Yazaki, Yoshiaki; Nagasuka, Kinuyo; Ohta, Eiji; Sakata, Makoto.

In: Plant and Cell Physiology, Vol. 25, No. 8, 12.1984, p. 1403-1409.

Research output: Contribution to journalArticle

Torimitsu, K, Yazaki, Y, Nagasuka, K, Ohta, E & Sakata, M 1984, 'Effect of external pH on the cytoplasmic and vacuolar phs in mung bean root-tip cells: A 31P nuclear magnetic resonance study', Plant and Cell Physiology, vol. 25, no. 8, pp. 1403-1409.
Torimitsu, Keiichi ; Yazaki, Yoshiaki ; Nagasuka, Kinuyo ; Ohta, Eiji ; Sakata, Makoto. / Effect of external pH on the cytoplasmic and vacuolar phs in mung bean root-tip cells : A 31P nuclear magnetic resonance study. In: Plant and Cell Physiology. 1984 ; Vol. 25, No. 8. pp. 1403-1409.
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