Effect of fasudil on Rho-kinase and nephropathy in subtotally nephrectomized spontaneously hypertensive rats

Takeshi Kanda, Shu Wakino, Koichi Hayashi, Koichiro Honma, Yuri Ozawa, Takao Saruta

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Background. Although Rho-kinase is reported to play an important role in vascular injury, the contribution of Rho-kinase to the progression of renal injury remains unestablished. Methods. We examined the effect of fasudil, a Rho-kinase inhibitor, on the progression of renal injury in subtotally nephrectomized spontaneously hypertensive rats (SHR). Rats were randomly assigned to three groups: sham-operated SHR: salt-loaded subtotally nephrectomized rats (SHR-subtotal nephrectomy); SHR-subtotal nephrectomy given fasudil for 6 weeks (SHR-subtotal nephrectomy + fasudil; 3 mg/kg/day). Renal morphologic and molecular analysis as well as urinary protein excretion was evaluated. Results. In SHR-subtotal nephrectomy treated with fasudil, systolic blood pressure was not significantly different from that in SHR-subtotal nephrectomy without fasudil (208 ± 8 mm Hg vs. 217 ± 14 mm Hg). Urinary protein excretion was markedly increased in SHR-subtotal nephrectomy (124 ± 16 mg/day), but this increase was significantly suppressed by fasudil (79 ± 12 mg/day). Renal histologic examination revealed that fasudil improved glomerular and tubulointerstitial injury scores with parallel amelioration of proliferating cell nuclear antigen-positive and ED-1-positive cell infiltration. Furthermore, Western blot analyses showed that both expression and activity of Rho-kinase were enhanced in SHR-subtotal nephrectomy, compared with those in SHR without nephrectomy, and fasudil suppressed Rho-kinase activity. Finally, fasudil up-regulated the expression of p27 kip1, a cyclin-dependent kinase inhibitor, and increased the p27 kip1 immunopositive cells in both glomeruli and tubulointerstitium with the use of immunohistochemistry. Conclusion. Rho-kinase pathway is involved in the pathogenesis of renal injury. Furthermore, the inhibition of Rho-kinase may constitute a therapeutic strategy for the treatment of renal injury in part through the p27kip1 up-regulation and the subsequent inhibition of cell proliferation and macrophage recruitment.

Original languageEnglish
Pages (from-to)2009-2019
Number of pages11
JournalKidney International
Volume64
Issue number6
DOIs
Publication statusPublished - 2003 Dec

Fingerprint

rho-Associated Kinases
Inbred SHR Rats
Nephrectomy
Kidney
Wounds and Injuries
fasudil
Cyclin-Dependent Kinase Inhibitor p27
Blood Pressure
Vascular System Injuries
Proliferating Cell Nuclear Antigen
Proteins
Up-Regulation
Salts
Western Blotting
Immunohistochemistry
Macrophages
Cell Proliferation

Keywords

  • Cell cycle
  • Fasudil
  • p27
  • Renal injury
  • Rho-kinase

ASJC Scopus subject areas

  • Nephrology

Cite this

Effect of fasudil on Rho-kinase and nephropathy in subtotally nephrectomized spontaneously hypertensive rats. / Kanda, Takeshi; Wakino, Shu; Hayashi, Koichi; Honma, Koichiro; Ozawa, Yuri; Saruta, Takao.

In: Kidney International, Vol. 64, No. 6, 12.2003, p. 2009-2019.

Research output: Contribution to journalArticle

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T1 - Effect of fasudil on Rho-kinase and nephropathy in subtotally nephrectomized spontaneously hypertensive rats

AU - Kanda, Takeshi

AU - Wakino, Shu

AU - Hayashi, Koichi

AU - Honma, Koichiro

AU - Ozawa, Yuri

AU - Saruta, Takao

PY - 2003/12

Y1 - 2003/12

N2 - Background. Although Rho-kinase is reported to play an important role in vascular injury, the contribution of Rho-kinase to the progression of renal injury remains unestablished. Methods. We examined the effect of fasudil, a Rho-kinase inhibitor, on the progression of renal injury in subtotally nephrectomized spontaneously hypertensive rats (SHR). Rats were randomly assigned to three groups: sham-operated SHR: salt-loaded subtotally nephrectomized rats (SHR-subtotal nephrectomy); SHR-subtotal nephrectomy given fasudil for 6 weeks (SHR-subtotal nephrectomy + fasudil; 3 mg/kg/day). Renal morphologic and molecular analysis as well as urinary protein excretion was evaluated. Results. In SHR-subtotal nephrectomy treated with fasudil, systolic blood pressure was not significantly different from that in SHR-subtotal nephrectomy without fasudil (208 ± 8 mm Hg vs. 217 ± 14 mm Hg). Urinary protein excretion was markedly increased in SHR-subtotal nephrectomy (124 ± 16 mg/day), but this increase was significantly suppressed by fasudil (79 ± 12 mg/day). Renal histologic examination revealed that fasudil improved glomerular and tubulointerstitial injury scores with parallel amelioration of proliferating cell nuclear antigen-positive and ED-1-positive cell infiltration. Furthermore, Western blot analyses showed that both expression and activity of Rho-kinase were enhanced in SHR-subtotal nephrectomy, compared with those in SHR without nephrectomy, and fasudil suppressed Rho-kinase activity. Finally, fasudil up-regulated the expression of p27 kip1, a cyclin-dependent kinase inhibitor, and increased the p27 kip1 immunopositive cells in both glomeruli and tubulointerstitium with the use of immunohistochemistry. Conclusion. Rho-kinase pathway is involved in the pathogenesis of renal injury. Furthermore, the inhibition of Rho-kinase may constitute a therapeutic strategy for the treatment of renal injury in part through the p27kip1 up-regulation and the subsequent inhibition of cell proliferation and macrophage recruitment.

AB - Background. Although Rho-kinase is reported to play an important role in vascular injury, the contribution of Rho-kinase to the progression of renal injury remains unestablished. Methods. We examined the effect of fasudil, a Rho-kinase inhibitor, on the progression of renal injury in subtotally nephrectomized spontaneously hypertensive rats (SHR). Rats were randomly assigned to three groups: sham-operated SHR: salt-loaded subtotally nephrectomized rats (SHR-subtotal nephrectomy); SHR-subtotal nephrectomy given fasudil for 6 weeks (SHR-subtotal nephrectomy + fasudil; 3 mg/kg/day). Renal morphologic and molecular analysis as well as urinary protein excretion was evaluated. Results. In SHR-subtotal nephrectomy treated with fasudil, systolic blood pressure was not significantly different from that in SHR-subtotal nephrectomy without fasudil (208 ± 8 mm Hg vs. 217 ± 14 mm Hg). Urinary protein excretion was markedly increased in SHR-subtotal nephrectomy (124 ± 16 mg/day), but this increase was significantly suppressed by fasudil (79 ± 12 mg/day). Renal histologic examination revealed that fasudil improved glomerular and tubulointerstitial injury scores with parallel amelioration of proliferating cell nuclear antigen-positive and ED-1-positive cell infiltration. Furthermore, Western blot analyses showed that both expression and activity of Rho-kinase were enhanced in SHR-subtotal nephrectomy, compared with those in SHR without nephrectomy, and fasudil suppressed Rho-kinase activity. Finally, fasudil up-regulated the expression of p27 kip1, a cyclin-dependent kinase inhibitor, and increased the p27 kip1 immunopositive cells in both glomeruli and tubulointerstitium with the use of immunohistochemistry. Conclusion. Rho-kinase pathway is involved in the pathogenesis of renal injury. Furthermore, the inhibition of Rho-kinase may constitute a therapeutic strategy for the treatment of renal injury in part through the p27kip1 up-regulation and the subsequent inhibition of cell proliferation and macrophage recruitment.

KW - Cell cycle

KW - Fasudil

KW - p27

KW - Renal injury

KW - Rho-kinase

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DO - 10.1046/j.1523-1755.2003.00300.x

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