Effect of glycerol on the affinity of DnaA protein for ATP in the presence of cardiolipin

Masakazu Hase, Yuichi Ishikawa, Kazuhisa Sekimizu, Tomofusa Tsuchiya, Tohru Mizushima

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Acidic phospholipids, such as cardiolipin, decrease the affinity of DnaA protein for adenine nucleotides and can activate the inactive form of DnaA protein in vitro. In this study, we examined the effect of glycerol on the affinity of DnaA protein for ATP in the presence of cardiolipin. High concentrations of glycerol (34%) restored the affinity of DnaA protein for ATP, which was decreased by cardiolipin. Glycerol inhibited the binding of cardiolipin with DnaA protein. Glycerol had little effect on membrane fluidity, which is essential for the interaction between cardiolipin and DnaA protein, whereas it increased the K(d) value of DnaA protein for ATP in the absence of cardiolipin. These results suggest that glycerol causes DnaA protein to become insensitive as to the interaction with cardiolipin by changing the conformation of the protein without altering the physical nature of the phospholipid.

Original languageEnglish
Pages (from-to)680-683
Number of pages4
JournalJournal of biochemistry
Volume123
Issue number4
DOIs
Publication statusPublished - 1998 Apr

Keywords

  • ATP-binding
  • Cardiolipin
  • DNA replication
  • DnaA protein
  • Glycerol

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Effect of glycerol on the affinity of DnaA protein for ATP in the presence of cardiolipin'. Together they form a unique fingerprint.

  • Cite this