Abstract
Acidic phospholipids, such as cardiolipin, decrease the affinity of DnaA protein for adenine nucleotides and can activate the inactive form of DnaA protein in vitro. In this study, we examined the effect of glycerol on the affinity of DnaA protein for ATP in the presence of cardiolipin. High concentrations of glycerol (34%) restored the affinity of DnaA protein for ATP, which was decreased by cardiolipin. Glycerol inhibited the binding of cardiolipin with DnaA protein. Glycerol had little effect on membrane fluidity, which is essential for the interaction between cardiolipin and DnaA protein, whereas it increased the K(d) value of DnaA protein for ATP in the absence of cardiolipin. These results suggest that glycerol causes DnaA protein to become insensitive as to the interaction with cardiolipin by changing the conformation of the protein without altering the physical nature of the phospholipid.
| Original language | English |
|---|---|
| Pages (from-to) | 680-683 |
| Number of pages | 4 |
| Journal | Journal of Biochemistry |
| Volume | 123 |
| Issue number | 4 |
| Publication status | Published - 1998 Apr |
| Externally published | Yes |
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Keywords
- ATP-binding
- Cardiolipin
- DNA replication
- DnaA protein
- Glycerol
ASJC Scopus subject areas
- Biochemistry
Cite this
Effect of glycerol on the affinity of DnaA protein for ATP in the presence of cardiolipin. / Hase, Masakazu; Ishikawa, Yuichi; Sekimizu, Kazuhisa; Tsuchiya, Tomofusa; Mizushima, Tohru.
In: Journal of Biochemistry, Vol. 123, No. 4, 04.1998, p. 680-683.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of glycerol on the affinity of DnaA protein for ATP in the presence of cardiolipin
AU - Hase, Masakazu
AU - Ishikawa, Yuichi
AU - Sekimizu, Kazuhisa
AU - Tsuchiya, Tomofusa
AU - Mizushima, Tohru
PY - 1998/4
Y1 - 1998/4
N2 - Acidic phospholipids, such as cardiolipin, decrease the affinity of DnaA protein for adenine nucleotides and can activate the inactive form of DnaA protein in vitro. In this study, we examined the effect of glycerol on the affinity of DnaA protein for ATP in the presence of cardiolipin. High concentrations of glycerol (34%) restored the affinity of DnaA protein for ATP, which was decreased by cardiolipin. Glycerol inhibited the binding of cardiolipin with DnaA protein. Glycerol had little effect on membrane fluidity, which is essential for the interaction between cardiolipin and DnaA protein, whereas it increased the K(d) value of DnaA protein for ATP in the absence of cardiolipin. These results suggest that glycerol causes DnaA protein to become insensitive as to the interaction with cardiolipin by changing the conformation of the protein without altering the physical nature of the phospholipid.
AB - Acidic phospholipids, such as cardiolipin, decrease the affinity of DnaA protein for adenine nucleotides and can activate the inactive form of DnaA protein in vitro. In this study, we examined the effect of glycerol on the affinity of DnaA protein for ATP in the presence of cardiolipin. High concentrations of glycerol (34%) restored the affinity of DnaA protein for ATP, which was decreased by cardiolipin. Glycerol inhibited the binding of cardiolipin with DnaA protein. Glycerol had little effect on membrane fluidity, which is essential for the interaction between cardiolipin and DnaA protein, whereas it increased the K(d) value of DnaA protein for ATP in the absence of cardiolipin. These results suggest that glycerol causes DnaA protein to become insensitive as to the interaction with cardiolipin by changing the conformation of the protein without altering the physical nature of the phospholipid.
KW - ATP-binding
KW - Cardiolipin
KW - DNA replication
KW - DnaA protein
KW - Glycerol
UR - http://www.scopus.com/inward/record.url?scp=0031968115&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031968115&partnerID=8YFLogxK
M3 - Article
C2 - 9538261
AN - SCOPUS:0031968115
VL - 123
SP - 680
EP - 683
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 4
ER -