TY - JOUR
T1 - Effect of herbal medicine Juzentaihoto on hepatic and intestinal heat shock gene expression requires intestinal microflora in mouse
AU - Kato, Miho
AU - Ishige, Atsushi
AU - Anjiki, Naoko
AU - Yamamoto, Masahiro
AU - Irie, Yoshifumi
AU - Taniyama, Mitsue
AU - Kibe, Ryoko
AU - Oka, Junichiro
AU - Benno, Yoshimi
AU - Watanabe, Kenji
PY - 2007/4/28
Y1 - 2007/4/28
N2 - Aim: To evaluate the role of intestinal microflora in the effects of multi-herbal medicine on gene expression in the gut and liver. Methods: The multi-herbal medicine Juzentaihoto (JTX) was administered to five germ-free mice and regular mice for 2 wk. Among the results of the comprehensive gene chip analysis of the intestine and liver, we featured heat shock proteins (HSPs) 70 and 105 because their gene expression changed only in the presence of microflora. Real-time RT-PCR was performed to confirm the expression levels of these HSP genes. To determine whether JTX acts directly on the HSP genes, sodium arsenite (SA) was used to induce the heat shock proteins directly. To examine the change of the intestinal microflora with administration of JTX, the terminal restriction fragment polymorphism (T-RFLP) method was used. To identify the changed bacteria, DNA sequencing was performed. Results: Heat shock protein gene expression, documented by gene chip and real-time RT-PCR, changed with the administration of JTX in the regular mice but not in the germ-free mice. JTX did not suppress the direct induction of the HSPs by SA. T-RFLP suggested that JTX decreased unculturable bacteria and increased Lactobacillus johnsoni. These data suggested that JTX changed the intestinal microflora which, in turn, changed HSP gene expression. Conclusion: Intestinal microflora affects multi-herbal product JTX on the gene expression in the gut and liver.
AB - Aim: To evaluate the role of intestinal microflora in the effects of multi-herbal medicine on gene expression in the gut and liver. Methods: The multi-herbal medicine Juzentaihoto (JTX) was administered to five germ-free mice and regular mice for 2 wk. Among the results of the comprehensive gene chip analysis of the intestine and liver, we featured heat shock proteins (HSPs) 70 and 105 because their gene expression changed only in the presence of microflora. Real-time RT-PCR was performed to confirm the expression levels of these HSP genes. To determine whether JTX acts directly on the HSP genes, sodium arsenite (SA) was used to induce the heat shock proteins directly. To examine the change of the intestinal microflora with administration of JTX, the terminal restriction fragment polymorphism (T-RFLP) method was used. To identify the changed bacteria, DNA sequencing was performed. Results: Heat shock protein gene expression, documented by gene chip and real-time RT-PCR, changed with the administration of JTX in the regular mice but not in the germ-free mice. JTX did not suppress the direct induction of the HSPs by SA. T-RFLP suggested that JTX decreased unculturable bacteria and increased Lactobacillus johnsoni. These data suggested that JTX changed the intestinal microflora which, in turn, changed HSP gene expression. Conclusion: Intestinal microflora affects multi-herbal product JTX on the gene expression in the gut and liver.
KW - Heat shock protein
KW - Herbal medicine
KW - Intestinal microflora
KW - Juzentaihoto
KW - Terminal restriction fragment length polymorphism
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U2 - 10.3748/wjg.v13.i16.2289
DO - 10.3748/wjg.v13.i16.2289
M3 - Article
C2 - 17511026
AN - SCOPUS:34249892572
VL - 13
SP - 2289
EP - 2297
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
SN - 1007-9327
IS - 16
ER -