Effect of herbimycin a on growth and pp60c-src activity in human colon tumor cell lines

R. Garcia, N. U. Parikh, Hideyuki Saya, G. E. Gallick

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The effect of herbimycin A, an ansamycin antibiotic which inhibits cellular transformation by retroviral tyrosine kinases, on the monolayer growth of seven colon tumor cell lines and one cell line established from normal colonic mucosa, CCL239, was examined. Each colon tumor cell line tested showed dose-dependent growth inhibition in response to herbimycin A. A 125 ng ml-1 dose of the antibiotic caused > 40% growth inhibition in all colon tumor cell lines after two cell doublings. In contrast, at similar herbimycin A concentrations only 12% inhibition was observed in 'normal' CCL239 cells. No major morphologic changes were observed at the light microscopic level in any of the tumor cell lines or CCL239 cells in response to treatment with herbimycin A. Studies using the HT29 colon adenocarcinoma cell line showed dosedependent inactivation of pp60c-src by herbimycin A, resulting in decreased autophosphorylation, enolase phosphorylation and steady-state levels, which correlated with cellular growth inhibition. Herbimycin A-induced reductions in pp60c-src kinase activity preceded changes in pp60c-src steady-state levels. Growth and pp60c-src inhibition were reversible following removal of herbimycin A from cell culture media. Our results suggest that regulation of pp60c-src tyrosine kinase activity may be important in growth control of colon tumor cells.

Original languageEnglish
Pages (from-to)1983-1989
Number of pages7
JournalOncogene
Volume6
Issue number11
Publication statusPublished - 1991 Nov
Externally publishedYes

Fingerprint

Tumor Cell Line
Human Activities
Colon
Growth
src-Family Kinases
Rifabutin
Anti-Bacterial Agents
Cell Line
Phosphopyruvate Hydratase
herbimycin
Protein-Tyrosine Kinases
Culture Media
Mucous Membrane
Adenocarcinoma
Cell Culture Techniques
Phosphorylation
Light
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Garcia, R., Parikh, N. U., Saya, H., & Gallick, G. E. (1991). Effect of herbimycin a on growth and pp60c-src activity in human colon tumor cell lines. Oncogene, 6(11), 1983-1989.

Effect of herbimycin a on growth and pp60c-src activity in human colon tumor cell lines. / Garcia, R.; Parikh, N. U.; Saya, Hideyuki; Gallick, G. E.

In: Oncogene, Vol. 6, No. 11, 11.1991, p. 1983-1989.

Research output: Contribution to journalArticle

Garcia, R, Parikh, NU, Saya, H & Gallick, GE 1991, 'Effect of herbimycin a on growth and pp60c-src activity in human colon tumor cell lines', Oncogene, vol. 6, no. 11, pp. 1983-1989.
Garcia, R. ; Parikh, N. U. ; Saya, Hideyuki ; Gallick, G. E. / Effect of herbimycin a on growth and pp60c-src activity in human colon tumor cell lines. In: Oncogene. 1991 ; Vol. 6, No. 11. pp. 1983-1989.
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