Effect of hesperetin on chaperone activity in selenite-induced cataract

Yosuke Nakazawa, Mikako Oka, Hiroomi Tamura, Makoto Takehana

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Background. Chaperone activity of α-crystallin in the lens works to prevent protein aggregation and is important to maintain the lens transparency. This study evaluated the effect of hesperetin on lens chaperone activity in selenite-induced cataracts. Methodology. Thirteen-day-old rats were divided into four groups. Animals were given hesperetin (groups G2 and G4) or vehicle (G1 and G3) on Days 0, 1, and 2. Rats in G3 and G4 were administered selenite subcutaneously 4 hours after the first hesperetin injection. On Days 2, 4, and 6, cataract grades were evaluated using slit-lamp biomicroscopy. The amount of a-crystallin and chaperone activity in water-soluble fraction were measured after animals sacrificed. Results. G3 on day 4 had developed significant cataract, as an average cataract grading of 4.6 ± 0.2. In contrast, G4 had less severe central opacities and lower stage cataracts than G3, as an average cataract grading of 2.4 ± 0.4. The a-crystallin levels in G3 lenses were lower than in G1, but the same as G4. Additionally, chaperone activity was weaker in G3 lenses than G1, but the same as in G4. Conclusions. Our results suggest that hesperetin can prevent the decreasing lens chaperone activity and a-crystallin water solubility by administered of selenite.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalOpen Medicine (Poland)
Volume11
Issue number1
DOIs
Publication statusPublished - 2016 Jun 1

Keywords

  • Anti-cataract effect
  • Cataract
  • Chaperone activity
  • Hesperetin
  • Selenite-induced cataract

ASJC Scopus subject areas

  • Medicine(all)

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