Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary

T. Miyazaki; K. Sueoka; A. M. Dharmarajan; S. J. Atlas; G. B. Bulkley; E. E. Wallach

doi:10.1530/jrf.0.0910207

Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary

T. Miyazaki, K. Sueoka, A. M. Dharmarajan, S. J. Atlas, G. B. Bulkley, E. E. Wallach

Research output: Contribution to journal › Article › peer-review

94 Citations (Scopus)

Abstract

The potential role of oxygen free radicals in hCG-induced ovulation was investigated using the free radical scavenging enzymes superoxide dismutase (SOD) and/or catalase with the in-vitro perfused rabbit ovary preparation. SOD (25 μg/ml) and SOD + catalase (25 μg/ml) significantly reduced the % of large follicles that ovulated during perfusion (P < 0.005). Neither maturity nor degeneration of ovulated ova and follicular oocytes was affected by SOD and/or catalase. Progesterone concentration in the perfusate was significantly increased in the SOD + catalase treatment group (P < 0.01). These results indicate a significant role for oxygen free radicals in the process of ovulation.

Original language	English
Pages (from-to)	207-212
Number of pages	6
Journal	Journal of Reproduction and Fertility
Volume	91
Issue number	1
DOIs	https://doi.org/10.1530/jrf.0.0910207
Publication status	Published - 1991
Externally published	Yes

Keywords

free radicals
ovulation
progesterone
superoxide dismutase

ASJC Scopus subject areas

Physiology
Embryology
Molecular Biology
Obstetrics and Gynaecology
Developmental Biology

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10.1530/jrf.0.0910207

Cite this

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abstract = "The potential role of oxygen free radicals in hCG-induced ovulation was investigated using the free radical scavenging enzymes superoxide dismutase (SOD) and/or catalase with the in-vitro perfused rabbit ovary preparation. SOD (25 μg/ml) and SOD + catalase (25 μg/ml) significantly reduced the % of large follicles that ovulated during perfusion (P < 0.005). Neither maturity nor degeneration of ovulated ova and follicular oocytes was affected by SOD and/or catalase. Progesterone concentration in the perfusate was significantly increased in the SOD + catalase treatment group (P < 0.01). These results indicate a significant role for oxygen free radicals in the process of ovulation.",

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AU - Miyazaki, T.

AU - Sueoka, K.

AU - Dharmarajan, A. M.

AU - Atlas, S. J.

AU - Bulkley, G. B.

AU - Wallach, E. E.

PY - 1991

Y1 - 1991

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AB - The potential role of oxygen free radicals in hCG-induced ovulation was investigated using the free radical scavenging enzymes superoxide dismutase (SOD) and/or catalase with the in-vitro perfused rabbit ovary preparation. SOD (25 μg/ml) and SOD + catalase (25 μg/ml) significantly reduced the % of large follicles that ovulated during perfusion (P < 0.005). Neither maturity nor degeneration of ovulated ova and follicular oocytes was affected by SOD and/or catalase. Progesterone concentration in the perfusate was significantly increased in the SOD + catalase treatment group (P < 0.01). These results indicate a significant role for oxygen free radicals in the process of ovulation.

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Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary

Abstract

Keywords

ASJC Scopus subject areas

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