Effect of inhibition of the ROCK isoform on RT2 malignant glioma cells

Nobuharu Inaba, Sho Ishizawa, Masaki Kimura, Kouki Fujioka, Michiko Watanabe, Toshiaki Shibasaki, Yoshinobu Manome

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15 Citations (Scopus)

Abstract

Background: Malignant glioma is one of the most intractable diseases in the human body. Rho-kinase (ROCK) is overexpressed and has been proposed as the main cause for the refractoriness of the disease. Since efficacious treatment is required, this study investigated the effect of inhibition of ROCK isoforms. Materials and Methods: The short hairpin RNA transcription vector was transfected into the RT2 rat glioma cell line and the characteristics of the cells were investigated. The effect of nimustine hydrochloride (ACNU) anti-neoplastic agent on cells was also measured. Results: Inhibition of ROCK isoforms did not alter cell growth. Cell cycle analysis revealed that ROCK1 down-regulation reduced the G0 phase population and ROCK2 down-regulation reduced the G2/M phase population. When ROCK1-down-regulated cells were exposed to ACNU, they demonstrated susceptibility to the agent. Conclusion: The roles of ROCK1 and ROCK2 may be different in glioma cells. Furthermore, the combination of ROCK1 down-regulation and an anti-neoplastic agent may be useful for the therapy of malignant glioma.

Original languageEnglish
Pages (from-to)3509-3514
Number of pages6
JournalAnticancer research
Volume30
Issue number9
Publication statusPublished - 2010 Sep

Keywords

  • ACNU
  • Cell cycle
  • Glioma
  • ROCK isoforms
  • shRNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Inaba, N., Ishizawa, S., Kimura, M., Fujioka, K., Watanabe, M., Shibasaki, T., & Manome, Y. (2010). Effect of inhibition of the ROCK isoform on RT2 malignant glioma cells. Anticancer research, 30(9), 3509-3514.