Effect of interleukin 6 (IL-6) on the differentiation and proliferation of murine and human hemopoietic progenitors

T. Suda, Y. Yamaguchi, J. Suda, Y. Miura, A. Okano, Y. Akiyama

Research output: Contribution to journalArticle

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Abstract

We examined the effect of human recombinant (r) interleukin 6 (IL-6) on the differentiation of murine and human hemopoietic progenitors. Human IL-6 supported colony formation by murine bone marrow cells. These colonies consisted of neutrophils and macrophages. Recombinant IL-6 was able to support multilineage colony formation by spleen cells from 5-fluorouracil (5-FU)-treated mice. These colonies consisted of >1 x 104 cells. Differential counts revealed large colonies exhibiting different combinations of cell lineages: neutrophils, macrophages, eosinophils, mast cells, and megakaryocytes. However, when blast cell colonies supported by interleukin 3 were replated into secondary dishes containing IL-6, they could differentiate into only neutrophils and macrophages. Single cells transferred from blast cell colonies formed only neutrophil/macrophage colonies. These results indicate that IL-6 had a direct effect on the growth and development of murine granulocyte-macrophage progenitors at a late stage and a significant effect on multipotential hemopoietic precursors that might be indirect through other cells. By contrast, human rIL-6 did not support colony formation by human bone marrow mononuclear cells. IL-6 may not show an independent acivity for human hemopoiesis of myeloid lineage. However, the synergistic activity of IL-6 remains to be clarified.

Original languageEnglish
Pages (from-to)891-895
Number of pages5
JournalExperimental Hematology
Volume16
Issue number11
Publication statusPublished - 1988

Fingerprint

Interleukin-6
Neutrophils
Macrophages
Bone Marrow Cells
Granulocyte-Macrophage Progenitor Cells
Megakaryocytes
Interleukin-3
Cell Lineage
Growth and Development
Eosinophils
Mast Cells
Fluorouracil
Spleen

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Suda, T., Yamaguchi, Y., Suda, J., Miura, Y., Okano, A., & Akiyama, Y. (1988). Effect of interleukin 6 (IL-6) on the differentiation and proliferation of murine and human hemopoietic progenitors. Experimental Hematology, 16(11), 891-895.

Effect of interleukin 6 (IL-6) on the differentiation and proliferation of murine and human hemopoietic progenitors. / Suda, T.; Yamaguchi, Y.; Suda, J.; Miura, Y.; Okano, A.; Akiyama, Y.

In: Experimental Hematology, Vol. 16, No. 11, 1988, p. 891-895.

Research output: Contribution to journalArticle

Suda, T, Yamaguchi, Y, Suda, J, Miura, Y, Okano, A & Akiyama, Y 1988, 'Effect of interleukin 6 (IL-6) on the differentiation and proliferation of murine and human hemopoietic progenitors', Experimental Hematology, vol. 16, no. 11, pp. 891-895.
Suda, T. ; Yamaguchi, Y. ; Suda, J. ; Miura, Y. ; Okano, A. ; Akiyama, Y. / Effect of interleukin 6 (IL-6) on the differentiation and proliferation of murine and human hemopoietic progenitors. In: Experimental Hematology. 1988 ; Vol. 16, No. 11. pp. 891-895.
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