Effect of interleukin 6 (IL-6) on the differentiation and proliferation of murine and human hemopoietic progenitors

T. Suda, Y. Yamaguchi, J. Suda, Y. Miura, A. Okano, Y. Akiyama

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Abstract

We examined the effect of human recombinant (r) interleukin 6 (IL-6) on the differentiation of murine and human hemopoietic progenitors. Human IL-6 supported colony formation by murine bone marrow cells. These colonies consisted of neutrophils and macrophages. Recombinant IL-6 was able to support multilineage colony formation by spleen cells from 5-fluorouracil (5-FU)-treated mice. These colonies consisted of >1 x 104 cells. Differential counts revealed large colonies exhibiting different combinations of cell lineages: neutrophils, macrophages, eosinophils, mast cells, and megakaryocytes. However, when blast cell colonies supported by interleukin 3 were replated into secondary dishes containing IL-6, they could differentiate into only neutrophils and macrophages. Single cells transferred from blast cell colonies formed only neutrophil/macrophage colonies. These results indicate that IL-6 had a direct effect on the growth and development of murine granulocyte-macrophage progenitors at a late stage and a significant effect on multipotential hemopoietic precursors that might be indirect through other cells. By contrast, human rIL-6 did not support colony formation by human bone marrow mononuclear cells. IL-6 may not show an independent acivity for human hemopoiesis of myeloid lineage. However, the synergistic activity of IL-6 remains to be clarified.

Original languageEnglish
Pages (from-to)891-895
Number of pages5
JournalExperimental Hematology
Volume16
Issue number11
Publication statusPublished - 1988 Dec 1

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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    Suda, T., Yamaguchi, Y., Suda, J., Miura, Y., Okano, A., & Akiyama, Y. (1988). Effect of interleukin 6 (IL-6) on the differentiation and proliferation of murine and human hemopoietic progenitors. Experimental Hematology, 16(11), 891-895.