Effect of isoproterenol on intracellular pH of the intercalated cells in the rabbit cortical collecting ducts

Matsuhiko Hayashi, Yasuyoshi Yamaji, Masahiro Iyori, Waichi Kitajima, Takao Saruta

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

To examine the mechanisms which regulate the functions of the intercalated cells (ICs) in the cortical collecting duct (CCD), the effect of isoproterenol on intracellular pH (pHi) of ICs was studied with the in vitro microperfused rabbit CCD, using the single cell pHi determination technique with fluorescent dye, 2′,7′-bis-(2-carboxyethyl)-5(and-6)carboxyfluorescein. The pHi of β-IC was significantly decreased with the addition of basolateral 10-6 M isoproterenol (7.21±0.04 to 7.05±0.04), whereas α-IC did not show any change. This response of β-IC to isoproterenol was dose-dependent and completely inhibited by the β-blockers, atenolol or propranolol. The addition of forskolin or 8-Br-cAMP mimicked the effects of isoproterenol, suggesting that the activation of adenylate cyclase induced the decrease in pHi. The rate of pHi changes after the Cl- removal from the perfusate, which is considered to reflect the activity of luminal anion exchanger, was significantly higher with isoproterenol (0.032±0.009 pH unit/s) than that in the control (0.023±0.009 pH unit/s). The present studies provide direct evidence for the regulation of β-IC function by β-adrenergic receptor; and the luminal Cl-/HCO3- exchanger was considered to be stimulated by β-agonist, directly.

Original languageEnglish
Pages (from-to)1153-1157
Number of pages5
JournalJournal of Clinical Investigation
Volume87
Issue number4
Publication statusPublished - 1991 Apr

Fingerprint

Isoproterenol
Rabbits
Chloride-Bicarbonate Antiporters
Atenolol
Colforsin
Fluorescent Dyes
Adenylyl Cyclases
Propranolol
Adrenergic Receptors
Anions

Keywords

  • Anion exchanger
  • Cyclic AMP
  • Forskolin
  • Microperfusion
  • Na/H exchanger

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effect of isoproterenol on intracellular pH of the intercalated cells in the rabbit cortical collecting ducts. / Hayashi, Matsuhiko; Yamaji, Yasuyoshi; Iyori, Masahiro; Kitajima, Waichi; Saruta, Takao.

In: Journal of Clinical Investigation, Vol. 87, No. 4, 04.1991, p. 1153-1157.

Research output: Contribution to journalArticle

Hayashi, M, Yamaji, Y, Iyori, M, Kitajima, W & Saruta, T 1991, 'Effect of isoproterenol on intracellular pH of the intercalated cells in the rabbit cortical collecting ducts', Journal of Clinical Investigation, vol. 87, no. 4, pp. 1153-1157.
Hayashi, Matsuhiko ; Yamaji, Yasuyoshi ; Iyori, Masahiro ; Kitajima, Waichi ; Saruta, Takao. / Effect of isoproterenol on intracellular pH of the intercalated cells in the rabbit cortical collecting ducts. In: Journal of Clinical Investigation. 1991 ; Vol. 87, No. 4. pp. 1153-1157.
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