Effect of low-dose paclitaxel and docetaxel on endothelial progenitor cells

Mariko Muta, Tomonori Yanagawa, Yoshimichi Sai, Shigehira Saji, Eiji Suzuki, Tomoyuki Aruga, Katsumasa Kuroi, Gaku Matsumoto, Masakazu Toi, Emi Nakashima

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14 Citations (Scopus)

Abstract

Objective: Bone marrow (BM)-derived endothelial progenitor cells (EPC) play an important role in neovascularization and tumor growth. It has been reported that docetaxel and paclitaxel inhibit angiogenesis, but the effect of docetaxel and paclitaxel on EPC-induced neovascularization has not been examined. We aimed to clarify the cytotoxic and inhibitory effects of these drugs on EPC. Methods: The effects of drugs on growth, tube formation, and migration of EPC were analyzed in vitro using a rat BM-derived EPC cell line (TR-BME). Fluorescence-labeled TR-BME cells were injected into tumor-bearing rats and accumulation at the tumor site was analyzed by fluorescence-activated cell sorting (FACS). Results: In in vitro cytotoxicity assays of these drugs in TR-BME, rat endothelial cell line TR-BBB and rat tumor cell line Walker 256, the IC50 values for TR-BME were higher than those for TR-BBB or Walker 256. Both drugs inhibited tube formation and migration of TR-BME at lower concentrations than the cytotoxic IC50. In vivo studies showed that a low dose of both drugs inhibited EPC accumulation at the tumor site in tumor-bearing rats, as determined by FACS, and caused a decrease in microvessel density. Conclusion: Docetaxel and paclitaxel directly inhibited EPC-initiated vasculogenesis at low (non-cytotoxic) concentrations, causing suppression of tumor growth.

Original languageEnglish
Pages (from-to)182-191
Number of pages10
JournalOncology
Volume77
Issue number3-4
DOIs
Publication statusPublished - 2009 Sep 1

Keywords

  • Docetaxel
  • Endothelial progenitor cells
  • Neovascularization
  • Paclitaxel
  • Vasculogenesis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Muta, M., Yanagawa, T., Sai, Y., Saji, S., Suzuki, E., Aruga, T., Kuroi, K., Matsumoto, G., Toi, M., & Nakashima, E. (2009). Effect of low-dose paclitaxel and docetaxel on endothelial progenitor cells. Oncology, 77(3-4), 182-191. https://doi.org/10.1159/000236016