Effect of low-intensity argon laser irradiation on mitochondrial respiration

Y. Morimoto, Tsunenori Arai, M. Kikuchi, S. Nakajima, H. Nakamura

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

We studied influences of low-intensity argon laser irradiation at various wavelengths on mitochondrial respiration in vitro. Isolated guinea pig liver mitochondria were suspended in an isotonic buffer solution (pH 7.4, 37°C). The mitochondrial suspension was introduced into a constant temperature reaction chamber in which an irradiation fiber, a thermocouple, an oxygen electrode, and a stirrer were installed. Under respiratory conditions of state 4, state 3, and uncoupled respiration, mitochondrial oxygen consumption was measured during low-intensity argon laser irradiations at 351 nm, 458 nm, and 514.5 nm. The 351 nm and the 458 nm irradiations at 200 mW inhibited uncoupled respiration by 19% and 11%, respectively, and the irradiation at 351 nm inhibited state 3 respiration as well by 10%. In contrast, the 514.5 nm irradiation enhanced both state 3 and uncoupled respiration by 6-7%. Temperature reference experiments indicated that the thermal effect alone could not account for the effects of laser irradiation on mitochondrial oxygen consumption. These results suggest that the 351 nm and the 458 nm laser irradiation may injure the mitochondrial inner membrane, while the 514.5 nm laser irradiation may slightly promote the rate of ATP synthesis.

Original languageEnglish
Pages (from-to)191-199
Number of pages9
JournalLasers in Surgery and Medicine
Volume15
Issue number2
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Argon
Respiration
Lasers
Oxygen Consumption
Isotonic Solutions
Temperature
Liver Mitochondrion
Mitochondrial Membranes
Suspensions
Buffers
Electrodes
Guinea Pigs
Hot Temperature
Adenosine Triphosphate
Oxygen

Keywords

  • mitochondria
  • state 3 respiration
  • state 4 respiration
  • uncoupled respiration

ASJC Scopus subject areas

  • Surgery

Cite this

Effect of low-intensity argon laser irradiation on mitochondrial respiration. / Morimoto, Y.; Arai, Tsunenori; Kikuchi, M.; Nakajima, S.; Nakamura, H.

In: Lasers in Surgery and Medicine, Vol. 15, No. 2, 1994, p. 191-199.

Research output: Contribution to journalArticle

Morimoto, Y, Arai, T, Kikuchi, M, Nakajima, S & Nakamura, H 1994, 'Effect of low-intensity argon laser irradiation on mitochondrial respiration', Lasers in Surgery and Medicine, vol. 15, no. 2, pp. 191-199. https://doi.org/10.1002/lsm.1900150207
Morimoto, Y. ; Arai, Tsunenori ; Kikuchi, M. ; Nakajima, S. ; Nakamura, H. / Effect of low-intensity argon laser irradiation on mitochondrial respiration. In: Lasers in Surgery and Medicine. 1994 ; Vol. 15, No. 2. pp. 191-199.
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