Effect of mechanical deformation on structure and function of polymorphonuclear leukocytes

Yuukou Kitagawa, Stephan F. Van Eeden, Darlene M. Redenbach, Maleki Daya, Blair A M Walker, Maria E. Klut, Barry R. Wiggs, James C. Hogg

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The present studies were designed to test the hypothesis that mechanical deformation of polymorphonuclear leukocytes (PMN) leads to functional changes that might influence their transit in the pulmonary capillaries. Human leukocytes were passed through 5- or 3-μm-pore polycarbonate filters under controlled conditions. Morphometric analysis showed that the majority of PMN were deformed and that this deformation persisted longer after filtration through 3-μm filters than through 5-μm filters (P < 0.05) but did not result in the cytoskeletal polarization characteristic of migrating cells. Flow cytometric studies of the filtered PMN showed that there was a transient increase in the cytosolic free Ca2+ concentration after both 3- and 5-μm filtration (P < 0.01) with an increase in F-actin content after 3-μm filtration (P < 0.05). Although L-selectin expression on PMN was not changed by either 5- or 3-μm filtration, CD18 and CD11b were increased by 3-μm filtration (P < 0.05). Priming of the PMN with N-formyl-methionyl-leucyl- phenylalanine (0.5 nM) before filtration resulted in an increase of CD11b by both 5 (P < 0.05)and 3-μm (P < 0.01) filtration. Neither 5- nor 3-μm filtration induced hydrogen peroxide production. We conclude that mechanical deformation of PMN, similar to what occurs in the pulmonary microvessels, induces both structural and functional changes in the cells, which might influence their passage through the pulmonary capillary bed.

Original languageEnglish
Pages (from-to)1397-1405
Number of pages9
JournalJournal of Applied Physiology
Volume82
Issue number5
Publication statusPublished - 1997 May
Externally publishedYes

Fingerprint

Neutrophils
polycarbonate
Lung
N-Formylmethionine Leucyl-Phenylalanine
L-Selectin
Microvessels
Hydrogen Peroxide
Actins
Leukocytes

Keywords

  • adhesion molecules
  • deformability
  • F-actin
  • neutrophils

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Kitagawa, Y., Van Eeden, S. F., Redenbach, D. M., Daya, M., Walker, B. A. M., Klut, M. E., ... Hogg, J. C. (1997). Effect of mechanical deformation on structure and function of polymorphonuclear leukocytes. Journal of Applied Physiology, 82(5), 1397-1405.

Effect of mechanical deformation on structure and function of polymorphonuclear leukocytes. / Kitagawa, Yuukou; Van Eeden, Stephan F.; Redenbach, Darlene M.; Daya, Maleki; Walker, Blair A M; Klut, Maria E.; Wiggs, Barry R.; Hogg, James C.

In: Journal of Applied Physiology, Vol. 82, No. 5, 05.1997, p. 1397-1405.

Research output: Contribution to journalArticle

Kitagawa, Y, Van Eeden, SF, Redenbach, DM, Daya, M, Walker, BAM, Klut, ME, Wiggs, BR & Hogg, JC 1997, 'Effect of mechanical deformation on structure and function of polymorphonuclear leukocytes', Journal of Applied Physiology, vol. 82, no. 5, pp. 1397-1405.
Kitagawa Y, Van Eeden SF, Redenbach DM, Daya M, Walker BAM, Klut ME et al. Effect of mechanical deformation on structure and function of polymorphonuclear leukocytes. Journal of Applied Physiology. 1997 May;82(5):1397-1405.
Kitagawa, Yuukou ; Van Eeden, Stephan F. ; Redenbach, Darlene M. ; Daya, Maleki ; Walker, Blair A M ; Klut, Maria E. ; Wiggs, Barry R. ; Hogg, James C. / Effect of mechanical deformation on structure and function of polymorphonuclear leukocytes. In: Journal of Applied Physiology. 1997 ; Vol. 82, No. 5. pp. 1397-1405.
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