Effect of Methylisobutyl Amiloride on [Na+]i, Reperfusion Arrhythmias, and Function in Ischemic Rat Hearts

Masato Tani, Ken Shinmura, Hiroshi Hasegawa, Yoshiro Nakamura

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

With 2 μM methylisobutyl amiloride (MIA), an inhibitor of Na+/H+ exchange, we tested the hypothesis that ion imbalance due to H+/Na+/Ca2+ exchange exacerbates reperfusion injury and arrhythmias. Isolated rat hearts were subjected to 25-min global ischemia and 30-min reperfusion. In the MIA-treated group, MIA was added throughout the perfusion protocol. Left ventricular pressure (LVP), arrhythmias, myocardial Na+ and K+ content, 45Ca2+ uptake, and the levels of energy metabolites were analyzed. The recovery of LV developed pressure (LVDP) and + dP/dt and - dP/dt were improved in the MIA group (53 vs. 80, 71 vs. 86, 77 vs. 94%: each p < 0.05). MIA inhibited the increase in Na+ content and the decrease in K+ content that occurred at the end of the ischemic phase and reduced 45Ca2+ uptake after reperfusion (28.6 vs. 17.1, 248 vs. 296, 2.79 vs. 1.36 μM/g dry weight of tissue; each p < 0.05). The incidence of ventricular tachycardia (VT) or ventricular fibrillation (VF) was lower in the MIA group [VT 11 of 20 (55%) vs. 4 of 20 (20%), p < 0.05; VF 13 of 20, (65%) vs. 6 of 20 (30%), 0.05 < p < 0.1], although the incidence of VF just escaped statistical significance. ATP level was higher in the MIA group after the ischemic phase and reperfusion (5.3 vs. 9.9, 12.3 vs. 14.7 μM/g dry weight of tissue; each p < 0.05). Our results suggest that MIA reduced reperfusion arrhythmias and improved functional recovery in isolated rat hearts subjected to global ischemia apparently by preserving high-energy phosphates during ischemia and by inhibiting Na+/H+ exchange, with attenuated cellular imbalance between Na+ and Ca2+.

Original languageEnglish
Pages (from-to)794-801
Number of pages8
JournalJournal of Cardiovascular Pharmacology
Volume27
Issue number6
Publication statusPublished - 1996

Fingerprint

Amiloride
Reperfusion
Cardiac Arrhythmias
Ventricular Fibrillation
Ischemia
Ventricular Tachycardia
Weights and Measures
Incidence
Ventricular Pressure
Reperfusion Injury
Perfusion
Adenosine Triphosphate
Phosphates
Ions
Pressure

Keywords

  • Intracellular Na
  • Left ventricular function
  • Na/Ca exchange
  • Na/H exchange
  • Reperfusion arrhythmias
  • Reperfusion injury

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pharmacology

Cite this

Effect of Methylisobutyl Amiloride on [Na+]i, Reperfusion Arrhythmias, and Function in Ischemic Rat Hearts. / Tani, Masato; Shinmura, Ken; Hasegawa, Hiroshi; Nakamura, Yoshiro.

In: Journal of Cardiovascular Pharmacology, Vol. 27, No. 6, 1996, p. 794-801.

Research output: Contribution to journalArticle

Tani, Masato ; Shinmura, Ken ; Hasegawa, Hiroshi ; Nakamura, Yoshiro. / Effect of Methylisobutyl Amiloride on [Na+]i, Reperfusion Arrhythmias, and Function in Ischemic Rat Hearts. In: Journal of Cardiovascular Pharmacology. 1996 ; Vol. 27, No. 6. pp. 794-801.
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AU - Hasegawa, Hiroshi

AU - Nakamura, Yoshiro

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AB - With 2 μM methylisobutyl amiloride (MIA), an inhibitor of Na+/H+ exchange, we tested the hypothesis that ion imbalance due to H+/Na+/Ca2+ exchange exacerbates reperfusion injury and arrhythmias. Isolated rat hearts were subjected to 25-min global ischemia and 30-min reperfusion. In the MIA-treated group, MIA was added throughout the perfusion protocol. Left ventricular pressure (LVP), arrhythmias, myocardial Na+ and K+ content, 45Ca2+ uptake, and the levels of energy metabolites were analyzed. The recovery of LV developed pressure (LVDP) and + dP/dt and - dP/dt were improved in the MIA group (53 vs. 80, 71 vs. 86, 77 vs. 94%: each p < 0.05). MIA inhibited the increase in Na+ content and the decrease in K+ content that occurred at the end of the ischemic phase and reduced 45Ca2+ uptake after reperfusion (28.6 vs. 17.1, 248 vs. 296, 2.79 vs. 1.36 μM/g dry weight of tissue; each p < 0.05). The incidence of ventricular tachycardia (VT) or ventricular fibrillation (VF) was lower in the MIA group [VT 11 of 20 (55%) vs. 4 of 20 (20%), p < 0.05; VF 13 of 20, (65%) vs. 6 of 20 (30%), 0.05 < p < 0.1], although the incidence of VF just escaped statistical significance. ATP level was higher in the MIA group after the ischemic phase and reperfusion (5.3 vs. 9.9, 12.3 vs. 14.7 μM/g dry weight of tissue; each p < 0.05). Our results suggest that MIA reduced reperfusion arrhythmias and improved functional recovery in isolated rat hearts subjected to global ischemia apparently by preserving high-energy phosphates during ischemia and by inhibiting Na+/H+ exchange, with attenuated cellular imbalance between Na+ and Ca2+.

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KW - Reperfusion arrhythmias

KW - Reperfusion injury

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