Effect of-opioid receptor agonist on the growth of non-small cell lung cancer (NSCLC) cells

N. Kuzumaki, A. Suzuki, M. Narita, T. Hosoya, A. Nagasawa, S. Imai, K. Yamamizu, H. Morita, H. Nagase, Y. Okada, H. J. Okano, J. K. Yamashita, H. Okano, T. Suzuki, M. Narita

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Background: It is becoming increasingly recognised that opioids are responsible for tumour growth. However, the effects of opioids on tumour growth have been controversial.Methods:The effects of-opioid receptor (KOR) agonist on the growth of non-small cell lung cancer (NSCLC) cells were assessed by a cell proliferation assay. Western blotting was performed to ascertain the mechanism by which treatment with KOR agonist suppresses tumour growth. Results: Addition of the selective KOR agonist U50,488H to gefitinib-sensitive (HCC827) and gefitinib-resistant (H1975) NSCLC cells produced a concentration-dependent decrease in their growth. These effects were abolished by co-treatment with the selective KOR antagonist nor-BNI. Furthermore, the growth-inhibitory effect of gefitinib in HCC827 cells was further enhanced by co-treatment with U50,488H. With regard to the inhibition of tumour growth, the addition of U50, 488H to H1975 cells produced a concentration-dependent decrease in phosphorylated- glycogen synthase kinase 3Β (p-GSK3Β).Conclusion:The present results showed that stimulation of KOR reduces the growth of gefitinib-resistant NSCLC cells through the activation of GSK3Β.

Original languageEnglish
Pages (from-to)1148-1152
Number of pages5
JournalBritish Journal of Cancer
Volume106
Issue number6
DOIs
Publication statusPublished - 2012 Mar 13

Keywords

  • gefitinib
  • non-small cell lung cancer
  • κ-opioid receptor

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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