Abstract
To investigate nerve injury by a photosensitization reaction ex vivo, we observed an uptake of talaporfin sodium into crayfish nerve and porcine phrenic nerve, and measured electrophysiological conduction velocity of the crayfish nerve during the reaction. We found the drug uptake of inside the perineurium was lower than that of outside in porcine phrenic nerve. The crayfish nerve was immersed into 20 µg/ml talaporfin sodium for 15 min and irradiated by a 663 nm laser light with 120 mW/cm2. Since we found the measured conduction velocity was decreased increasing the irradiation time, the nerve might not be resistant to the photosensitization reaction at atmospheric oxygen environment. It was reported that the phrenic nerve was intact when an electro blockade using photosensitization reaction was performed in vivo animal experiment. Low uptake of talaporfin sodium inside the perineurium and low oxygen partial pressure of nerve might be the mechanism to preserve phrenic nerve in vivo animal experiment.
| Original language | English |
|---|---|
| Pages (from-to) | 574-575 |
| Number of pages | 2 |
| Journal | Transactions of Japanese Society for Medical and Biological Engineering |
| Volume | 55 |
| Issue number | Proc |
| DOIs | |
| Publication status | Published - 2017 |
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Keywords
- Oxygen partial pressure
- Photosensitization reaction
- Phrenic nerve
- Talaporfin sodium
ASJC Scopus subject areas
- Biomedical Engineering
Cite this
Effect of photosensitization reaction on myelinated or unmyelinated nerve. / Takahashi, Haruka; Hamada, Risa; Ogawa, Emiyu; Arai, Tsunenori.
In: Transactions of Japanese Society for Medical and Biological Engineering, Vol. 55, No. Proc, 2017, p. 574-575.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of photosensitization reaction on myelinated or unmyelinated nerve
AU - Takahashi, Haruka
AU - Hamada, Risa
AU - Ogawa, Emiyu
AU - Arai, Tsunenori
PY - 2017
Y1 - 2017
N2 - To investigate nerve injury by a photosensitization reaction ex vivo, we observed an uptake of talaporfin sodium into crayfish nerve and porcine phrenic nerve, and measured electrophysiological conduction velocity of the crayfish nerve during the reaction. We found the drug uptake of inside the perineurium was lower than that of outside in porcine phrenic nerve. The crayfish nerve was immersed into 20 µg/ml talaporfin sodium for 15 min and irradiated by a 663 nm laser light with 120 mW/cm2. Since we found the measured conduction velocity was decreased increasing the irradiation time, the nerve might not be resistant to the photosensitization reaction at atmospheric oxygen environment. It was reported that the phrenic nerve was intact when an electro blockade using photosensitization reaction was performed in vivo animal experiment. Low uptake of talaporfin sodium inside the perineurium and low oxygen partial pressure of nerve might be the mechanism to preserve phrenic nerve in vivo animal experiment.
AB - To investigate nerve injury by a photosensitization reaction ex vivo, we observed an uptake of talaporfin sodium into crayfish nerve and porcine phrenic nerve, and measured electrophysiological conduction velocity of the crayfish nerve during the reaction. We found the drug uptake of inside the perineurium was lower than that of outside in porcine phrenic nerve. The crayfish nerve was immersed into 20 µg/ml talaporfin sodium for 15 min and irradiated by a 663 nm laser light with 120 mW/cm2. Since we found the measured conduction velocity was decreased increasing the irradiation time, the nerve might not be resistant to the photosensitization reaction at atmospheric oxygen environment. It was reported that the phrenic nerve was intact when an electro blockade using photosensitization reaction was performed in vivo animal experiment. Low uptake of talaporfin sodium inside the perineurium and low oxygen partial pressure of nerve might be the mechanism to preserve phrenic nerve in vivo animal experiment.
KW - Oxygen partial pressure
KW - Photosensitization reaction
KW - Phrenic nerve
KW - Talaporfin sodium
UR - http://www.scopus.com/inward/record.url?scp=85029835192&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029835192&partnerID=8YFLogxK
U2 - 10.11239/jsmbe.55Annual.574
DO - 10.11239/jsmbe.55Annual.574
M3 - Article
VL - 55
SP - 574
EP - 575
JO - BME = Bio medical engineering / henshu, Nihon ME Gakkai
JF - BME = Bio medical engineering / henshu, Nihon ME Gakkai
SN - 1347-443X
IS - Proc
ER -