Effect of Post-Ischemic Hypothermia on Spinal Cord Damage induced by Transient Ischemic Insult in Rabbits

Koji Tsutsumi, Toshihiko Ueda, Hideyuki Shimizu, Kenichi Hashizume, Yoshimi Iino, Shiaki Kawada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: The effect of post-ischemic mild hypothermia applied immediately after induced transient ischemia on the extent of neuronal damage to the spinal cord was investigated in rabbit. Subjects and Methods: A 15-minute period of transient abdominal aortic occlusion for spinal cord ischemia at a rectal temperature of 37.3 ± 0.3°C was performed just below the left renal vein via median laparotomy. Three groups of rabbits were investigated; Group 1 (n = 8) subjected to ischemia and reperfused at the same temperature for 7 hours, Group 2 (n = 8) also subjected to ischemia and then to 6 hours of systemic hypothermia (32.5 ± 0.5°C), and Group 3 (n = 8) non-ischemic controls. All the rabbits in Group 1 and Group 2 were sacrificed at 1 week after ischemic injury. Spinal cord sections were examined microscopically to determine the extent of ischemic neuronal damage. Results: The mean modified Tarlov's score at 1 week after ischemic injury was 0.5 ± 0.8 in Group 1, whereas it was 4.4 ± 1.4 (p <.001) in Group 2. The mean total number of surviving neurons within examined sections of the spinal cord was significantly greater in Group 2 than in Group 1 (Group 1: 8 ± 66.1 vs Group 2: 300.9 ± 154.1, p <.001). Conclusions: Post-ischemic hypothermia induced immediately after reperfusion significantly reduced ischemia-induced neuronal damage in rabbit.

Original languageEnglish
Pages (from-to)359-365
Number of pages7
JournalJapanese Journal of Thoracic and Cardiovascular Surgery
Volume50
Issue number9
Publication statusPublished - 2002 Sep

Fingerprint

Hypothermia
Spinal Cord
Ischemia
Rabbits
Spinal Cord Ischemia
Induced Hypothermia
Temperature
Renal Veins
Wounds and Injuries
Laparotomy
Reperfusion
Neurons

Keywords

  • Aortic surgery
  • Post-ischemic hypothermia
  • Spinal protection

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Effect of Post-Ischemic Hypothermia on Spinal Cord Damage induced by Transient Ischemic Insult in Rabbits. / Tsutsumi, Koji; Ueda, Toshihiko; Shimizu, Hideyuki; Hashizume, Kenichi; Iino, Yoshimi; Kawada, Shiaki.

In: Japanese Journal of Thoracic and Cardiovascular Surgery, Vol. 50, No. 9, 09.2002, p. 359-365.

Research output: Contribution to journalArticle

Tsutsumi, Koji ; Ueda, Toshihiko ; Shimizu, Hideyuki ; Hashizume, Kenichi ; Iino, Yoshimi ; Kawada, Shiaki. / Effect of Post-Ischemic Hypothermia on Spinal Cord Damage induced by Transient Ischemic Insult in Rabbits. In: Japanese Journal of Thoracic and Cardiovascular Surgery. 2002 ; Vol. 50, No. 9. pp. 359-365.
@article{356acf2d60304e849d895899d8e22a79,
title = "Effect of Post-Ischemic Hypothermia on Spinal Cord Damage induced by Transient Ischemic Insult in Rabbits",
abstract = "Objective: The effect of post-ischemic mild hypothermia applied immediately after induced transient ischemia on the extent of neuronal damage to the spinal cord was investigated in rabbit. Subjects and Methods: A 15-minute period of transient abdominal aortic occlusion for spinal cord ischemia at a rectal temperature of 37.3 ± 0.3°C was performed just below the left renal vein via median laparotomy. Three groups of rabbits were investigated; Group 1 (n = 8) subjected to ischemia and reperfused at the same temperature for 7 hours, Group 2 (n = 8) also subjected to ischemia and then to 6 hours of systemic hypothermia (32.5 ± 0.5°C), and Group 3 (n = 8) non-ischemic controls. All the rabbits in Group 1 and Group 2 were sacrificed at 1 week after ischemic injury. Spinal cord sections were examined microscopically to determine the extent of ischemic neuronal damage. Results: The mean modified Tarlov's score at 1 week after ischemic injury was 0.5 ± 0.8 in Group 1, whereas it was 4.4 ± 1.4 (p <.001) in Group 2. The mean total number of surviving neurons within examined sections of the spinal cord was significantly greater in Group 2 than in Group 1 (Group 1: 8 ± 66.1 vs Group 2: 300.9 ± 154.1, p <.001). Conclusions: Post-ischemic hypothermia induced immediately after reperfusion significantly reduced ischemia-induced neuronal damage in rabbit.",
keywords = "Aortic surgery, Post-ischemic hypothermia, Spinal protection",
author = "Koji Tsutsumi and Toshihiko Ueda and Hideyuki Shimizu and Kenichi Hashizume and Yoshimi Iino and Shiaki Kawada",
year = "2002",
month = "9",
language = "English",
volume = "50",
pages = "359--365",
journal = "General Thoracic and Cardiovascular Surgery",
issn = "1863-6705",
publisher = "Springer Japan",
number = "9",

}

TY - JOUR

T1 - Effect of Post-Ischemic Hypothermia on Spinal Cord Damage induced by Transient Ischemic Insult in Rabbits

AU - Tsutsumi, Koji

AU - Ueda, Toshihiko

AU - Shimizu, Hideyuki

AU - Hashizume, Kenichi

AU - Iino, Yoshimi

AU - Kawada, Shiaki

PY - 2002/9

Y1 - 2002/9

N2 - Objective: The effect of post-ischemic mild hypothermia applied immediately after induced transient ischemia on the extent of neuronal damage to the spinal cord was investigated in rabbit. Subjects and Methods: A 15-minute period of transient abdominal aortic occlusion for spinal cord ischemia at a rectal temperature of 37.3 ± 0.3°C was performed just below the left renal vein via median laparotomy. Three groups of rabbits were investigated; Group 1 (n = 8) subjected to ischemia and reperfused at the same temperature for 7 hours, Group 2 (n = 8) also subjected to ischemia and then to 6 hours of systemic hypothermia (32.5 ± 0.5°C), and Group 3 (n = 8) non-ischemic controls. All the rabbits in Group 1 and Group 2 were sacrificed at 1 week after ischemic injury. Spinal cord sections were examined microscopically to determine the extent of ischemic neuronal damage. Results: The mean modified Tarlov's score at 1 week after ischemic injury was 0.5 ± 0.8 in Group 1, whereas it was 4.4 ± 1.4 (p <.001) in Group 2. The mean total number of surviving neurons within examined sections of the spinal cord was significantly greater in Group 2 than in Group 1 (Group 1: 8 ± 66.1 vs Group 2: 300.9 ± 154.1, p <.001). Conclusions: Post-ischemic hypothermia induced immediately after reperfusion significantly reduced ischemia-induced neuronal damage in rabbit.

AB - Objective: The effect of post-ischemic mild hypothermia applied immediately after induced transient ischemia on the extent of neuronal damage to the spinal cord was investigated in rabbit. Subjects and Methods: A 15-minute period of transient abdominal aortic occlusion for spinal cord ischemia at a rectal temperature of 37.3 ± 0.3°C was performed just below the left renal vein via median laparotomy. Three groups of rabbits were investigated; Group 1 (n = 8) subjected to ischemia and reperfused at the same temperature for 7 hours, Group 2 (n = 8) also subjected to ischemia and then to 6 hours of systemic hypothermia (32.5 ± 0.5°C), and Group 3 (n = 8) non-ischemic controls. All the rabbits in Group 1 and Group 2 were sacrificed at 1 week after ischemic injury. Spinal cord sections were examined microscopically to determine the extent of ischemic neuronal damage. Results: The mean modified Tarlov's score at 1 week after ischemic injury was 0.5 ± 0.8 in Group 1, whereas it was 4.4 ± 1.4 (p <.001) in Group 2. The mean total number of surviving neurons within examined sections of the spinal cord was significantly greater in Group 2 than in Group 1 (Group 1: 8 ± 66.1 vs Group 2: 300.9 ± 154.1, p <.001). Conclusions: Post-ischemic hypothermia induced immediately after reperfusion significantly reduced ischemia-induced neuronal damage in rabbit.

KW - Aortic surgery

KW - Post-ischemic hypothermia

KW - Spinal protection

UR - http://www.scopus.com/inward/record.url?scp=0036717985&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036717985&partnerID=8YFLogxK

M3 - Article

C2 - 12382401

AN - SCOPUS:0036717985

VL - 50

SP - 359

EP - 365

JO - General Thoracic and Cardiovascular Surgery

JF - General Thoracic and Cardiovascular Surgery

SN - 1863-6705

IS - 9

ER -