Effect of quinine solutions on intracellular Ca2+ levels in neuro-2a cells - Conventional physiological method for the evaluation of bitterness

Tomoko Nakamura, Takeshi Akiyoshi, Naoko Tanaka, Kazumasa Shinozuka, Sumio Matzno, Toshikatsu Nakabayashi, Kenji Matsuyama, Makoto Kashiwayanagi, Takahiro Uchida

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The purpose of the present study was to examine the effect of quinine on intracellular Ca2+ ([Ca2+]i) levels in cultured neuro-2a cells, and to investigate the possibility of using [Ca 2+]i levels to predict the bitterness of quinine solutions. [Ca2+]i levels in neuro-2a cells increased following stimulation by quinine in a concentration-related manner. There was a good linear correlationship between the quinine-induced increase in [Ca 2+]i levels increase and the bitterness scores of the quinine solutions as assessed in human gustatory sensation tests (r 2=0.918). The quinine-induced increase in [Ca2+] i levels was inhibited by thapsigargin (an inhibitor of the Ca 2+ pump into intracellular stores), U73122 (an inhibitor of phospholipase C) and ω-conotoxin (an N-type Ca2+-channel blocker), but not by nifedipine (an L-type Ca2+-channel blocker).

Original languageEnglish
Pages (from-to)1637-1640
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume26
Issue number11
DOIs
Publication statusPublished - 2003 Nov
Externally publishedYes

Keywords

  • Bitterness
  • Intracellular Ca
  • Neuro-2a cell
  • Quinine
  • Taste sensor
  • Thapsigargin

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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