Effect of quinine solutions on intracellular Ca2+ levels in neuro-2a cells - Conventional physiological method for the evaluation of bitterness

Tomoko Nakamura; Takeshi Akiyoshi; Naoko Tanaka; Kazumasa Shinozuka; Sumio Matzno; Toshikatsu Nakabayashi; Kenji Matsuyama; Makoto Kashiwayanagi; Takahiro Uchida

doi:10.1248/bpb.26.1637

Effect of quinine solutions on intracellular Ca²⁺ levels in neuro-2a cells - Conventional physiological method for the evaluation of bitterness

Tomoko Nakamura, Takeshi Akiyoshi, Naoko Tanaka, Kazumasa Shinozuka, Sumio Matzno, Toshikatsu Nakabayashi, Kenji Matsuyama, Makoto Kashiwayanagi, Takahiro Uchida

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The purpose of the present study was to examine the effect of quinine on intracellular Ca²⁺ ([Ca²⁺]_i) levels in cultured neuro-2a cells, and to investigate the possibility of using [Ca ²⁺]_i levels to predict the bitterness of quinine solutions. [Ca²⁺]_i levels in neuro-2a cells increased following stimulation by quinine in a concentration-related manner. There was a good linear correlationship between the quinine-induced increase in [Ca ²⁺]_i levels increase and the bitterness scores of the quinine solutions as assessed in human gustatory sensation tests (r ²=0.918). The quinine-induced increase in [Ca²⁺] _i levels was inhibited by thapsigargin (an inhibitor of the Ca ²⁺ pump into intracellular stores), U73122 (an inhibitor of phospholipase C) and ω-conotoxin (an N-type Ca²⁺-channel blocker), but not by nifedipine (an L-type Ca²⁺-channel blocker).

Original language	English
Pages (from-to)	1637-1640
Number of pages	4
Journal	Biological and Pharmaceutical Bulletin
Volume	26
Issue number	11
DOIs	https://doi.org/10.1248/bpb.26.1637
Publication status	Published - 2003 Nov
Externally published	Yes

Keywords

Bitterness
Intracellular Ca
Neuro-2a cell
Quinine
Taste sensor
Thapsigargin

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

Access to Document

10.1248/bpb.26.1637

Cite this

Nakamura, T., Akiyoshi, T., Tanaka, N., Shinozuka, K., Matzno, S., Nakabayashi, T., Matsuyama, K., Kashiwayanagi, M., & Uchida, T. (2003). Effect of quinine solutions on intracellular Ca²⁺ levels in neuro-2a cells - Conventional physiological method for the evaluation of bitterness. Biological and Pharmaceutical Bulletin, 26(11), 1637-1640. https://doi.org/10.1248/bpb.26.1637

Nakamura, T, Akiyoshi, T, Tanaka, N, Shinozuka, K, Matzno, S, Nakabayashi, T, Matsuyama, K, Kashiwayanagi, M & Uchida, T 2003, 'Effect of quinine solutions on intracellular Ca²⁺ levels in neuro-2a cells - Conventional physiological method for the evaluation of bitterness', Biological and Pharmaceutical Bulletin, vol. 26, no. 11, pp. 1637-1640. https://doi.org/10.1248/bpb.26.1637

@article{24bb0cadd02544e88751b7cde7adfd99,

title = "Effect of quinine solutions on intracellular Ca2+ levels in neuro-2a cells - Conventional physiological method for the evaluation of bitterness",

abstract = "The purpose of the present study was to examine the effect of quinine on intracellular Ca2+ ([Ca2+]i) levels in cultured neuro-2a cells, and to investigate the possibility of using [Ca 2+]i levels to predict the bitterness of quinine solutions. [Ca2+]i levels in neuro-2a cells increased following stimulation by quinine in a concentration-related manner. There was a good linear correlationship between the quinine-induced increase in [Ca 2+]i levels increase and the bitterness scores of the quinine solutions as assessed in human gustatory sensation tests (r 2=0.918). The quinine-induced increase in [Ca2+] i levels was inhibited by thapsigargin (an inhibitor of the Ca 2+ pump into intracellular stores), U73122 (an inhibitor of phospholipase C) and ω-conotoxin (an N-type Ca2+-channel blocker), but not by nifedipine (an L-type Ca2+-channel blocker).",

keywords = "Bitterness, Intracellular Ca, Neuro-2a cell, Quinine, Taste sensor, Thapsigargin",

author = "Tomoko Nakamura and Takeshi Akiyoshi and Naoko Tanaka and Kazumasa Shinozuka and Sumio Matzno and Toshikatsu Nakabayashi and Kenji Matsuyama and Makoto Kashiwayanagi and Takahiro Uchida",

year = "2003",

month = nov,

doi = "10.1248/bpb.26.1637",

language = "English",

volume = "26",

pages = "1637--1640",

journal = "Biological and Pharmaceutical Bulletin",

issn = "0918-6158",

publisher = "Pharmaceutical Society of Japan",

number = "11",

}

TY - JOUR

T1 - Effect of quinine solutions on intracellular Ca2+ levels in neuro-2a cells - Conventional physiological method for the evaluation of bitterness

AU - Nakamura, Tomoko

AU - Akiyoshi, Takeshi

AU - Tanaka, Naoko

AU - Shinozuka, Kazumasa

AU - Matzno, Sumio

AU - Nakabayashi, Toshikatsu

AU - Matsuyama, Kenji

AU - Kashiwayanagi, Makoto

AU - Uchida, Takahiro

PY - 2003/11

Y1 - 2003/11

N2 - The purpose of the present study was to examine the effect of quinine on intracellular Ca2+ ([Ca2+]i) levels in cultured neuro-2a cells, and to investigate the possibility of using [Ca 2+]i levels to predict the bitterness of quinine solutions. [Ca2+]i levels in neuro-2a cells increased following stimulation by quinine in a concentration-related manner. There was a good linear correlationship between the quinine-induced increase in [Ca 2+]i levels increase and the bitterness scores of the quinine solutions as assessed in human gustatory sensation tests (r 2=0.918). The quinine-induced increase in [Ca2+] i levels was inhibited by thapsigargin (an inhibitor of the Ca 2+ pump into intracellular stores), U73122 (an inhibitor of phospholipase C) and ω-conotoxin (an N-type Ca2+-channel blocker), but not by nifedipine (an L-type Ca2+-channel blocker).

AB - The purpose of the present study was to examine the effect of quinine on intracellular Ca2+ ([Ca2+]i) levels in cultured neuro-2a cells, and to investigate the possibility of using [Ca 2+]i levels to predict the bitterness of quinine solutions. [Ca2+]i levels in neuro-2a cells increased following stimulation by quinine in a concentration-related manner. There was a good linear correlationship between the quinine-induced increase in [Ca 2+]i levels increase and the bitterness scores of the quinine solutions as assessed in human gustatory sensation tests (r 2=0.918). The quinine-induced increase in [Ca2+] i levels was inhibited by thapsigargin (an inhibitor of the Ca 2+ pump into intracellular stores), U73122 (an inhibitor of phospholipase C) and ω-conotoxin (an N-type Ca2+-channel blocker), but not by nifedipine (an L-type Ca2+-channel blocker).

KW - Bitterness

KW - Intracellular Ca

KW - Neuro-2a cell

KW - Quinine

KW - Taste sensor

KW - Thapsigargin

UR - http://www.scopus.com/inward/record.url?scp=25844510593&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25844510593&partnerID=8YFLogxK

U2 - 10.1248/bpb.26.1637

DO - 10.1248/bpb.26.1637

M3 - Article

C2 - 14600419

AN - SCOPUS:25844510593

SN - 0918-6158

VL - 26

SP - 1637

EP - 1640

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

IS - 11

ER -