Effect of refrigeration of blood samples in lithium-heparin tubes on QuantiFERON TB Gold Plus test result

Yoshifumi Uwamino, Akiko Sakai, Tomoyasu Nishimura, Masayo Noguchi, Shunsuke Uno, Hiroshi Fujiwara, Masaaki Mori, Masatoshi Wakui, Mitsuru Murata, Naoki Hasegawa

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The QuantiFERON TB Gold Plus (QFT-Plus) test is a newly approved interferon-gamma releasing assay test for detecting latent tuberculosis. Although blood samples for QFT test can be refrigerated for 48 h in lithium-heparin tubes according to package inserts, no published data are available on the effects of sample refrigeration on the test results. We conducted a clinical study that aimed to elucidate whether sample refrigeration for 48 h affects QFT-Plus test results. We collected 2 blood samples each from 40 participants for QFT-Plus; one sample was refrigerated before incubation for QFT-Plus assay, while the other sample was incubated soon after collection and treated as control. After comparing QFT-Plus test results of refrigerated samples and control samples, the concordance rate and kappa coefficient between them were 95% and 0.90, respectively. Thus, blood samples for QFT-Plus test can be refrigerated for 48 h in lithium-heparin tubes without influencing the test results.

Original languageEnglish
Pages (from-to)312-314
Number of pages3
JournalJournal of Infection and Chemotherapy
Volume26
Issue number3
DOIs
Publication statusPublished - 2020 Mar

Keywords

  • Latent tuberculosis infection
  • QuantiFERON TB Gold Plus
  • Refrigeration
  • Sample storage

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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