Effect of retinol palmitate as a treatment for dry eye: A cytological evaluation

Tadao K. Kobayashi, Kazuo Tsubota, Etsuko Takamura, Mitsuru Sawa, Yuichi Ohashi, Masahiko Usui

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Vitamin A is known to regulate the proliferation and differentiation of corneal epithelial cells and preserved conjunctival goblet cells and has been used in the treatment of disease of the eye such as dry eye and superior limbic keratoconjunctivitis for some time. This study was undertaken in order to evaluate the efficacy of retinol palmitate aqueous ophthalmic solution under development for the treatment of dry eye failing to respond to the conventional therapy with artificial tears or cornea-protective drugs. Retinol palmitate ophthalmic solution was applied repeatedly for 4 consecutive weeks. Before and after instillation therapy, brush cytology (Cytobrush-S®) was performed and cytodiagnosis was made for keratinized cells, nonkeratinized cells, goblet cells and inflammatory cells on samples prepared using an automated smear apparatus (ThinPrep®). In dry eye, an increase in goblet cells (1.3 ± 2.6 → 2.1 ± 1.8 cells/slides), a decrease in keratinized cells (11.2 ± 16.5 → 5.2 ± 10.9 cells/300 cells) and, hence, an increase in nonkeratinized cells (287.3 ± 16.6 → 293.4 ± 11.4/300 cells) were found after treatment with retinol palmitate. As to inflammatory cells, there was no change from the protreatment baseline (1.4 ± 1.4 → 1.4 ± 1.3 cells/300 cells). These results demonstrate that brush cytology suggests the efficacy of retinol palmitate ophthalmic solution in dry eye treatment.

Original languageEnglish
Pages (from-to)358-361
Number of pages4
JournalOphthalmologica
Volume211
Issue number6
Publication statusPublished - 1997 Nov
Externally publishedYes

Fingerprint

Goblet Cells
Ophthalmic Solutions
Therapeutics
Cell Biology
retinol palmitate
Cytodiagnosis
Keratoconjunctivitis
Protective Agents
Eye Diseases
Vitamin A
Cornea
Epithelial Cells

Keywords

  • Brush cytology
  • Dry eye
  • ThinPrep process
  • Vitamin A

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Kobayashi, T. K., Tsubota, K., Takamura, E., Sawa, M., Ohashi, Y., & Usui, M. (1997). Effect of retinol palmitate as a treatment for dry eye: A cytological evaluation. Ophthalmologica, 211(6), 358-361.

Effect of retinol palmitate as a treatment for dry eye : A cytological evaluation. / Kobayashi, Tadao K.; Tsubota, Kazuo; Takamura, Etsuko; Sawa, Mitsuru; Ohashi, Yuichi; Usui, Masahiko.

In: Ophthalmologica, Vol. 211, No. 6, 11.1997, p. 358-361.

Research output: Contribution to journalArticle

Kobayashi, TK, Tsubota, K, Takamura, E, Sawa, M, Ohashi, Y & Usui, M 1997, 'Effect of retinol palmitate as a treatment for dry eye: A cytological evaluation', Ophthalmologica, vol. 211, no. 6, pp. 358-361.
Kobayashi TK, Tsubota K, Takamura E, Sawa M, Ohashi Y, Usui M. Effect of retinol palmitate as a treatment for dry eye: A cytological evaluation. Ophthalmologica. 1997 Nov;211(6):358-361.
Kobayashi, Tadao K. ; Tsubota, Kazuo ; Takamura, Etsuko ; Sawa, Mitsuru ; Ohashi, Yuichi ; Usui, Masahiko. / Effect of retinol palmitate as a treatment for dry eye : A cytological evaluation. In: Ophthalmologica. 1997 ; Vol. 211, No. 6. pp. 358-361.
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