Effect of secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide-1 (SLURP-1) on airway epithelial cells

Osamu Narumoto, Yuichi Niikura, Satoshi Ishii, Hirofumi Morihara, Saki Okashiro, Takashi Nakahari, Takashi Nakano, Hitoshi Matsumura, Chikao Shimamoto, Yasuhiro Moriwaki, Hidemi Misawa, Naohide Yamashita, Takahide Nagase, Koichiro Kawashima, Naomi Yamashita

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Acetylcholine (ACh) exerts various anti-inflammatory effects through α7 nicotinic ACh receptors (nAChRs). We have previously shown that secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide-1 (SLURP-1), a positive allosteric modulator of α7 nAChR signaling, is down-regulated both in an animal model of asthma and in human epithelial cells treated with an inflammatory cytokine related to asthma. Our aim of this study was to explore the effect of SLURP-1, signal through α7 nAChR, in the pathophysiology of airway inflammation. Cytokine production was examined using human epithelial cells. Ciliary beat frequency of murine trachea was measured using a high speed camera. The IL-6 and TNF-α production by human epithelial cells was augmented by siRNA of SLURP-1 and α7 nicotinic ACh receptor. The cytokine production was also dose-dependently suppressed by human recombinant SLURP-1 (rSLURP-1). The ciliary beat frequency and amplitude of murine epithelial cells were augmented by PNU282987, a selective α7 nAChR agonist. Those findings suggested that SLURP-1 and stimulus through α7 nicotinic ACh receptors actively controlled asthmatic condition by stimulating ciliary beating and also by suppressing airway inflammation.

Original languageEnglish
Pages (from-to)175-179
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume438
Issue number1
DOIs
Publication statusPublished - 2013 Aug 16

Keywords

  • Acetylcholine
  • Asthma
  • Bronchial epithelial cells
  • Ciliary beating
  • Mucociliary transport
  • Nicotinic receptors

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Effect of secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide-1 (SLURP-1) on airway epithelial cells'. Together they form a unique fingerprint.

  • Cite this

    Narumoto, O., Niikura, Y., Ishii, S., Morihara, H., Okashiro, S., Nakahari, T., Nakano, T., Matsumura, H., Shimamoto, C., Moriwaki, Y., Misawa, H., Yamashita, N., Nagase, T., Kawashima, K., & Yamashita, N. (2013). Effect of secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide-1 (SLURP-1) on airway epithelial cells. Biochemical and Biophysical Research Communications, 438(1), 175-179. https://doi.org/10.1016/j.bbrc.2013.07.048