TY - JOUR
T1 - Effect of serum factors on the differentiation of a colon cancer cell line Caco-2
AU - Hara, Ayumi
AU - Serizawa, Hiroshi
AU - Saito, Hidetsugu
AU - Iwao, Yasushi
AU - Watanabe, Tetsu
AU - Tsuchiya, Masaharu
PY - 1992/1/1
Y1 - 1992/1/1
N2 - A human colon cancer cell line Caco-2 undergoes spontaneous enterocytic differentiation under the standard culture condition. We examined the changes in the proliferative activity of Caco-2 cells during the differentiation, and the effect of serum factors on this spontaneous differentiation. Caco-2 cells cultured in FCS-containing media exhibited the differentiation phenotypes such as dome formation and increase of ALP activity after the 6th day of culture. Cell cycle analysis showed a marked decrease in S phase cell population during the cell differentiation. When Caco-2 cells were cultured in FCS free media, the cell population of S phaseremained unchanged during culture period and the differentiation phemotypes were not induced. The addition of FCS to Caco-2 cell cultured in the absence of FCS resulted in the rapid induction of the differentiation phenotypes. These results suggest that differentiation of Caco-2 cells is closely related to the reduction of cell proliferation and requires some factors in FCS.
AB - A human colon cancer cell line Caco-2 undergoes spontaneous enterocytic differentiation under the standard culture condition. We examined the changes in the proliferative activity of Caco-2 cells during the differentiation, and the effect of serum factors on this spontaneous differentiation. Caco-2 cells cultured in FCS-containing media exhibited the differentiation phenotypes such as dome formation and increase of ALP activity after the 6th day of culture. Cell cycle analysis showed a marked decrease in S phase cell population during the cell differentiation. When Caco-2 cells were cultured in FCS free media, the cell population of S phaseremained unchanged during culture period and the differentiation phemotypes were not induced. The addition of FCS to Caco-2 cell cultured in the absence of FCS resulted in the rapid induction of the differentiation phenotypes. These results suggest that differentiation of Caco-2 cells is closely related to the reduction of cell proliferation and requires some factors in FCS.
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U2 - 10.11405/nisshoshi1964.89.588
DO - 10.11405/nisshoshi1964.89.588
M3 - Article
C2 - 1578803
AN - SCOPUS:0026521863
SN - 0446-6586
VL - 89
SP - 588
EP - 595
JO - Journal of Japanese Society of Gastroenterology
JF - Journal of Japanese Society of Gastroenterology
IS - 3
ER -
