Effect of surface character on proliferation and adhesion of l929 fibroblast

Atsushi Nagat, Shogo Miyata, Mario Saito, Jun Komotori, Shinnosuke Obi, Yoji Naiviba, Tadayoshi Koyaivia

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this study, the effects of Fine Particle Peemng (FPP) treatment on adhesion and proliferation in L929 fibroblast were investigated. FPP treatments were performed on the Ti-6A1-4V alloy specimen by using Ti0 2 (T series) , glass beads (G series) and Alumina (Al series) shot particle. The polished specimen (P series) was also prepaid. Cell culture tests on the specimen were carried out with L929 fibroblast after 6 hours, 1, 3 and 5 days culture. Cell proliferation was evaluated by counting the number of cells by MTT assay. Cell adhesion strength was also measured by counting the number of cells detached by fluid-induced shear stress. The FPP treated surface by using shot particle of varied materials, which have different surface character. The Ti02 series showed good cell proliferation. This was because the protein adsorption of Ti0 2 series encourage cell adhesion. The detachment cells on the specimen were observed after share stress loading. The FPP treated series showed good cell adhesion. This was because the microscopically surface topography encourage cell adhesion.

Original languageEnglish
Pages (from-to)544-551
Number of pages8
JournalNihon Kikai Gakkai Ronbunshu, A Hen/Transactions of the Japan Society of Mechanical Engineers, Part A
Volume77
Issue number775
Publication statusPublished - 2011 Dec 1

Keywords

  • Biological Engineering
  • Biomaterials
  • Cell Culture
  • Fine Particle Peening
  • L929 Fibroblast
  • Protein Adsorption
  • Surface Improvement
  • Surfacetopograpy
  • Titarnum

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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