Effect of the antitubulin drug oryzalin on the encystation of Entamoeba invadens

Asao Makioka, Masahiro Kumagai, Hiroshi Ohtomo, Seiki Kobayashi, Tsutomu Takeuchi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have recently demonstrated that the antimicrotubule drug oryzalin inhibits the growth of Entamoeba invadens as well as E. histolytica, the former being more resistant to the drug than the latter, and that effective doses of oryzalin are higher for Entamoeba than for the other parasitic protozoa examined thus far. The aim of the present study was to examine the effect of oryzalin on the encystation of E. invadens using an axenic encystation system in vitro. Oryzalin inhibited the encystation of E. invadens strain IP-1 in a dose-dependent manner. The addition of oryzalin after the induction of encystation was also inhibitory for encystation and cyst maturation. Trophozoites incubated for 1 day in encystation medium with oryzalin did not encyst after removal of the drug. Although trophozoites grown in the presence of 300 μM oryzalin for 2 days did not encyst after their transfer to encystation medium containing the same concentration of drug, a number of trophozoites survived for at least 3 days. In contrast, trophozoites grown in the absence of oryzalin neither survived nor encysted after their transfer to encystation medium supplemented with the drug, which suggests that pretreatment of trophozoites with oryzalin contributes to their continued survival as trophozoites, i.e., without their transforming into cysts, in encystation medium. Trophozoites grown with oryzalin did encyst after their transfer to encystation medium without the drug. Accumulation of trophozoites in the mitotic phase was observed after culture with oryzalin. When cysts prepared at day 1 of encystation, most of which were mononucleate, were reincubated in the presence of oryzalin for an additional 2 days, inhibition of their maturation was observed. Thus, oryzalin is a potent mitotic-phase inhibitor of E. invadens and may become a useful tool for studies on the relationship between the cell cycle and encystation of this parasite.

Original languageEnglish
Pages (from-to)625-629
Number of pages5
JournalParasitology Research
Volume86
Issue number8
Publication statusPublished - 2000

Fingerprint

Entamoeba
oryzalin
encystment
Trophozoites
trophozoites
drugs
Pharmaceutical Preparations
Cysts
Entamoeba invadens
Entamoeba histolytica
dosage

ASJC Scopus subject areas

  • Parasitology

Cite this

Makioka, A., Kumagai, M., Ohtomo, H., Kobayashi, S., & Takeuchi, T. (2000). Effect of the antitubulin drug oryzalin on the encystation of Entamoeba invadens. Parasitology Research, 86(8), 625-629.

Effect of the antitubulin drug oryzalin on the encystation of Entamoeba invadens. / Makioka, Asao; Kumagai, Masahiro; Ohtomo, Hiroshi; Kobayashi, Seiki; Takeuchi, Tsutomu.

In: Parasitology Research, Vol. 86, No. 8, 2000, p. 625-629.

Research output: Contribution to journalArticle

Makioka, A, Kumagai, M, Ohtomo, H, Kobayashi, S & Takeuchi, T 2000, 'Effect of the antitubulin drug oryzalin on the encystation of Entamoeba invadens', Parasitology Research, vol. 86, no. 8, pp. 625-629.
Makioka A, Kumagai M, Ohtomo H, Kobayashi S, Takeuchi T. Effect of the antitubulin drug oryzalin on the encystation of Entamoeba invadens. Parasitology Research. 2000;86(8):625-629.
Makioka, Asao ; Kumagai, Masahiro ; Ohtomo, Hiroshi ; Kobayashi, Seiki ; Takeuchi, Tsutomu. / Effect of the antitubulin drug oryzalin on the encystation of Entamoeba invadens. In: Parasitology Research. 2000 ; Vol. 86, No. 8. pp. 625-629.
@article{dc5f6e0d355c4f5e9db76089b12e1c9e,
title = "Effect of the antitubulin drug oryzalin on the encystation of Entamoeba invadens",
abstract = "We have recently demonstrated that the antimicrotubule drug oryzalin inhibits the growth of Entamoeba invadens as well as E. histolytica, the former being more resistant to the drug than the latter, and that effective doses of oryzalin are higher for Entamoeba than for the other parasitic protozoa examined thus far. The aim of the present study was to examine the effect of oryzalin on the encystation of E. invadens using an axenic encystation system in vitro. Oryzalin inhibited the encystation of E. invadens strain IP-1 in a dose-dependent manner. The addition of oryzalin after the induction of encystation was also inhibitory for encystation and cyst maturation. Trophozoites incubated for 1 day in encystation medium with oryzalin did not encyst after removal of the drug. Although trophozoites grown in the presence of 300 μM oryzalin for 2 days did not encyst after their transfer to encystation medium containing the same concentration of drug, a number of trophozoites survived for at least 3 days. In contrast, trophozoites grown in the absence of oryzalin neither survived nor encysted after their transfer to encystation medium supplemented with the drug, which suggests that pretreatment of trophozoites with oryzalin contributes to their continued survival as trophozoites, i.e., without their transforming into cysts, in encystation medium. Trophozoites grown with oryzalin did encyst after their transfer to encystation medium without the drug. Accumulation of trophozoites in the mitotic phase was observed after culture with oryzalin. When cysts prepared at day 1 of encystation, most of which were mononucleate, were reincubated in the presence of oryzalin for an additional 2 days, inhibition of their maturation was observed. Thus, oryzalin is a potent mitotic-phase inhibitor of E. invadens and may become a useful tool for studies on the relationship between the cell cycle and encystation of this parasite.",
author = "Asao Makioka and Masahiro Kumagai and Hiroshi Ohtomo and Seiki Kobayashi and Tsutomu Takeuchi",
year = "2000",
language = "English",
volume = "86",
pages = "625--629",
journal = "Zeitschrift fur Parasitenkunde",
issn = "0044-3255",
publisher = "Springer Verlag",
number = "8",

}

TY - JOUR

T1 - Effect of the antitubulin drug oryzalin on the encystation of Entamoeba invadens

AU - Makioka, Asao

AU - Kumagai, Masahiro

AU - Ohtomo, Hiroshi

AU - Kobayashi, Seiki

AU - Takeuchi, Tsutomu

PY - 2000

Y1 - 2000

N2 - We have recently demonstrated that the antimicrotubule drug oryzalin inhibits the growth of Entamoeba invadens as well as E. histolytica, the former being more resistant to the drug than the latter, and that effective doses of oryzalin are higher for Entamoeba than for the other parasitic protozoa examined thus far. The aim of the present study was to examine the effect of oryzalin on the encystation of E. invadens using an axenic encystation system in vitro. Oryzalin inhibited the encystation of E. invadens strain IP-1 in a dose-dependent manner. The addition of oryzalin after the induction of encystation was also inhibitory for encystation and cyst maturation. Trophozoites incubated for 1 day in encystation medium with oryzalin did not encyst after removal of the drug. Although trophozoites grown in the presence of 300 μM oryzalin for 2 days did not encyst after their transfer to encystation medium containing the same concentration of drug, a number of trophozoites survived for at least 3 days. In contrast, trophozoites grown in the absence of oryzalin neither survived nor encysted after their transfer to encystation medium supplemented with the drug, which suggests that pretreatment of trophozoites with oryzalin contributes to their continued survival as trophozoites, i.e., without their transforming into cysts, in encystation medium. Trophozoites grown with oryzalin did encyst after their transfer to encystation medium without the drug. Accumulation of trophozoites in the mitotic phase was observed after culture with oryzalin. When cysts prepared at day 1 of encystation, most of which were mononucleate, were reincubated in the presence of oryzalin for an additional 2 days, inhibition of their maturation was observed. Thus, oryzalin is a potent mitotic-phase inhibitor of E. invadens and may become a useful tool for studies on the relationship between the cell cycle and encystation of this parasite.

AB - We have recently demonstrated that the antimicrotubule drug oryzalin inhibits the growth of Entamoeba invadens as well as E. histolytica, the former being more resistant to the drug than the latter, and that effective doses of oryzalin are higher for Entamoeba than for the other parasitic protozoa examined thus far. The aim of the present study was to examine the effect of oryzalin on the encystation of E. invadens using an axenic encystation system in vitro. Oryzalin inhibited the encystation of E. invadens strain IP-1 in a dose-dependent manner. The addition of oryzalin after the induction of encystation was also inhibitory for encystation and cyst maturation. Trophozoites incubated for 1 day in encystation medium with oryzalin did not encyst after removal of the drug. Although trophozoites grown in the presence of 300 μM oryzalin for 2 days did not encyst after their transfer to encystation medium containing the same concentration of drug, a number of trophozoites survived for at least 3 days. In contrast, trophozoites grown in the absence of oryzalin neither survived nor encysted after their transfer to encystation medium supplemented with the drug, which suggests that pretreatment of trophozoites with oryzalin contributes to their continued survival as trophozoites, i.e., without their transforming into cysts, in encystation medium. Trophozoites grown with oryzalin did encyst after their transfer to encystation medium without the drug. Accumulation of trophozoites in the mitotic phase was observed after culture with oryzalin. When cysts prepared at day 1 of encystation, most of which were mononucleate, were reincubated in the presence of oryzalin for an additional 2 days, inhibition of their maturation was observed. Thus, oryzalin is a potent mitotic-phase inhibitor of E. invadens and may become a useful tool for studies on the relationship between the cell cycle and encystation of this parasite.

UR - http://www.scopus.com/inward/record.url?scp=0033835136&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033835136&partnerID=8YFLogxK

M3 - Article

C2 - 10952260

AN - SCOPUS:0033835136

VL - 86

SP - 625

EP - 629

JO - Zeitschrift fur Parasitenkunde

JF - Zeitschrift fur Parasitenkunde

SN - 0044-3255

IS - 8

ER -