Effect of vitamin K2 on cortical and cancellous bone mass and hepatic lipids in rats with combined methionine-choline deficiency

Jun Iwamoto, Azusa Seki, Yoshihiro Sato, Hideo Matsumoto, Tsuyoshi Takeda, James K. Yeh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The present study examined changes of cancellous and cortical bone in rats with combined methionine-choline deficiency (MCD). In addition, the effects of vitamin K2 on cortical and cancellous bone mass and hepatic lipids were investigated in rats with MCD. Six-week-old male Sprague-Dawley rats were randomized into three groups of ten, including an age-matched control (standard diet) group, an MCD diet group, and an MCD diet. +. vitamin K2 (menatetrenone at 30. mg/kg/d orally, 5 times a week) group. After the one-month experimental period, histomorphometric analysis was performed on cortical and cancellous bone from the tibial diaphysis and proximal metaphysis, respectively, while histological examination of the liver was performed after staining with hematoxylin and eosin and Oil Red O. MCD rats displayed weight loss, diffuse and centrilobular fatty changes of the liver, and a decrease of the cancellous bone volume per tissue volume (BV/TV) and percent cortical area (Ct Ar) as a result of decreased trabecular, periosteal, and endocortical bone formation along with increased trabecular and endocortical bone resorption. Administration of vitamin K2 to rats with MCD attenuated weight loss, accelerated the decrease of cancellous BV/TV due to an increase of bone remodeling, and ameliorated the decrease of percent Ct Ar by increasing periosteal and endocortical bone formation. Vitamin K2 administration also prevented MCD-induced diffuse fatty change of the liver. These findings suggest a beneficial effect of vitamin K2 on cortical bone mass and hepatic lipid metabolism in rats with MCD. The loss of cancellous bone mass could possibly have been due to re-distribution of minerals to cortical bone.

Original languageEnglish
Pages (from-to)1015-1021
Number of pages7
JournalBone
Volume48
Issue number5
DOIs
Publication statusPublished - 2011 May 1

Fingerprint

Choline Deficiency
Vitamin K 2
Methionine
Lipids
Liver
Fatty Liver
Diet
Osteogenesis
Weight Loss
Diaphyses
Cancellous Bone
Cortical Bone
Bone Remodeling
Hematoxylin
Eosine Yellowish-(YS)
Bone Resorption
Lipid Metabolism
Minerals
Sprague Dawley Rats
Demography

Keywords

  • Bone histomorphometry
  • Choline
  • Fatty liver
  • Methionine
  • Rat
  • Vitamin k2

ASJC Scopus subject areas

  • Physiology
  • Endocrinology, Diabetes and Metabolism
  • Histology

Cite this

Effect of vitamin K2 on cortical and cancellous bone mass and hepatic lipids in rats with combined methionine-choline deficiency. / Iwamoto, Jun; Seki, Azusa; Sato, Yoshihiro; Matsumoto, Hideo; Takeda, Tsuyoshi; Yeh, James K.

In: Bone, Vol. 48, No. 5, 01.05.2011, p. 1015-1021.

Research output: Contribution to journalArticle

Iwamoto, Jun ; Seki, Azusa ; Sato, Yoshihiro ; Matsumoto, Hideo ; Takeda, Tsuyoshi ; Yeh, James K. / Effect of vitamin K2 on cortical and cancellous bone mass and hepatic lipids in rats with combined methionine-choline deficiency. In: Bone. 2011 ; Vol. 48, No. 5. pp. 1015-1021.
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