Effect of vitamin K2 on cortical and cancellous bones in orchidectomized young rats

Jun Iwamoto, Tsuyoshi Takeda, James K. Yeh, Shoichi Ichimura, Yoshiaki Toyama

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Objectives: The purpose of the present study was to compare the effect of vitamin K2 on cortical and cancellous bones in orchidectomized young rats. Methods: Forty male Sprague-Dawley rats, 6 weeks of age, were randomized by stratified weight method into four groups with 10 rats in each group: baseline controls (BLC), age-matched controls (AMC), orchidectomy (ORX), and ORX+vitamin K2 administration (K). Vitamin K2 (menatetrenone) was administered subcutaneously twice a week at dose of 30 mg/kg each. The experimental period was 8 weeks, and cortical and cancellous bone histomorphometry was performed on the tibial shaft and the proximal tibia, respectively. Results: Cortical area (Ct Ar) and cancellous bone volume (BV/TV) were significantly greater in the AMC group than in the BLC group. Ct Ar was significantly lower in the ORX group than in the AMC group, and cancellous BV/TV was also significantly lower in the ORX group than in the AMC group as a result of significantly increased eroded surface (ES/BS). Although Ct Ar in the ORX+K group did not differ significantly from that in the ORX group, cancellous BV/TV was significantly greater in the ORX+K group than in the ORX group, but still significantly lower than in the AMC group. This protective effect of vitamin K2 on cancellous bone was attributable to normalizing increased ES/BS. Conclusions: Vitamin K2 appears to act more strongly on cancellous bone than on cortical bone in ORX young rats. High dose vitamin K2 could partially prevent the reduction of cancellous bone gain by normalizing raised bone resorption in ORX young rats.

Original languageEnglish
Pages (from-to)19-27
Number of pages9
JournalMaturitas
Volume44
Issue number1
DOIs
Publication statusPublished - 2003 Jan 30

Keywords

  • Bone histomorphometry
  • Bone resorption
  • Male osteoporosis
  • Orchidectomy
  • Vitamin K

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Obstetrics and Gynaecology

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