Effect of wall shear stress on macromolecule uptake into cultured endothelial cells

Susumu Kudo, Kenji Ikezawa, Shinji Matsumura, Mariko Ikeda, Kotaro Oka, Kazuo Tanishita

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The purpose of this study is to reveal macromolecule uptake route (intercellular or intracellular) in the endothelial cell layer, and to examine the effect of wall shear stress on the uptake. After 48 hour exposure to shear stress, the endothelial cell layer on coverslips were incubated at 37°C for 60 minutes in PBS containing tetramethylrhodamine isothiocyanate conjugated albumin (TRITC-albumin). Thereafter, the uptake of albumin and the shape of endothelial cells were observed by a confocal laser scanning microscope (CLSM). Albumin is found in intracellular region, not in intercellular region. The albumin uptake depends on imposed shear stress. At 10 dyn/cm2, the albumin uptake showed a 1.3 folds increase. The albumin uptake decreases with increasing shear stress, and minimum uptake is quarter of the control value at 60 dyn/cm2. This shear dependence of uptake is an unique feature of the cell and may play a key role for the controlling mechanism of endothelial cells.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalNihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
Volume64
Issue number618
DOIs
Publication statusPublished - 1998 Feb

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Keywords

  • Albumin
  • Bio-fluid mechanics
  • Biological engineering
  • Endothelial cell
  • Macromolecule uptake
  • Shear flow

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Mechanical Engineering

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