Effect on DNA topology by DnaA protein, the initiation factor of chromosomal DNA replication in Escherichia coli

Tohru Mizushima, Tsutomu Katayama, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We examined effects on supercoiled DNA topology of DnaA protein, the initiator protein of chromosomal DNA replication in Escherichia coli. The activity was identified in an analysis of plasmid DNA incubated with DnaA protein and DNA topoisomerase I. In Superose 12 gel filtration chromatography, the activity coeluted with DnaA protein. Incubation of DnaA protein with DNA at temperatures over 24 °C was required for this activity, which was observed with either oriC plasmid or the replicative form I of φX174 with no DnaA box. As binding of ATP or ADP to DnaA protein prevented the activity of DnaA protein on DNA topology, binding of the adenine nucleotide may regulate the activity.

Original languageEnglish
Pages (from-to)11512-11516
Number of pages5
JournalBiochemistry
Volume35
Issue number35
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Peptide Initiation Factors
DNA Replication
Escherichia coli
Topology
DNA
Proteins
Plasmids
Superhelical DNA
Type I DNA Topoisomerase
Adenine Nucleotides
Chromatography
Adenosine Diphosphate
Gel Chromatography
Adenosine Triphosphate
Gels
Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

Effect on DNA topology by DnaA protein, the initiation factor of chromosomal DNA replication in Escherichia coli. / Mizushima, Tohru; Katayama, Tsutomu; Sekimizu, Kazuhisa.

In: Biochemistry, Vol. 35, No. 35, 1996, p. 11512-11516.

Research output: Contribution to journalArticle

Mizushima, Tohru ; Katayama, Tsutomu ; Sekimizu, Kazuhisa. / Effect on DNA topology by DnaA protein, the initiation factor of chromosomal DNA replication in Escherichia coli. In: Biochemistry. 1996 ; Vol. 35, No. 35. pp. 11512-11516.
@article{8e3d25281cf14a27a63805b5badab748,
title = "Effect on DNA topology by DnaA protein, the initiation factor of chromosomal DNA replication in Escherichia coli",
abstract = "We examined effects on supercoiled DNA topology of DnaA protein, the initiator protein of chromosomal DNA replication in Escherichia coli. The activity was identified in an analysis of plasmid DNA incubated with DnaA protein and DNA topoisomerase I. In Superose 12 gel filtration chromatography, the activity coeluted with DnaA protein. Incubation of DnaA protein with DNA at temperatures over 24 °C was required for this activity, which was observed with either oriC plasmid or the replicative form I of φX174 with no DnaA box. As binding of ATP or ADP to DnaA protein prevented the activity of DnaA protein on DNA topology, binding of the adenine nucleotide may regulate the activity.",
author = "Tohru Mizushima and Tsutomu Katayama and Kazuhisa Sekimizu",
year = "1996",
doi = "10.1021/bi953088f",
language = "English",
volume = "35",
pages = "11512--11516",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "35",

}

TY - JOUR

T1 - Effect on DNA topology by DnaA protein, the initiation factor of chromosomal DNA replication in Escherichia coli

AU - Mizushima, Tohru

AU - Katayama, Tsutomu

AU - Sekimizu, Kazuhisa

PY - 1996

Y1 - 1996

N2 - We examined effects on supercoiled DNA topology of DnaA protein, the initiator protein of chromosomal DNA replication in Escherichia coli. The activity was identified in an analysis of plasmid DNA incubated with DnaA protein and DNA topoisomerase I. In Superose 12 gel filtration chromatography, the activity coeluted with DnaA protein. Incubation of DnaA protein with DNA at temperatures over 24 °C was required for this activity, which was observed with either oriC plasmid or the replicative form I of φX174 with no DnaA box. As binding of ATP or ADP to DnaA protein prevented the activity of DnaA protein on DNA topology, binding of the adenine nucleotide may regulate the activity.

AB - We examined effects on supercoiled DNA topology of DnaA protein, the initiator protein of chromosomal DNA replication in Escherichia coli. The activity was identified in an analysis of plasmid DNA incubated with DnaA protein and DNA topoisomerase I. In Superose 12 gel filtration chromatography, the activity coeluted with DnaA protein. Incubation of DnaA protein with DNA at temperatures over 24 °C was required for this activity, which was observed with either oriC plasmid or the replicative form I of φX174 with no DnaA box. As binding of ATP or ADP to DnaA protein prevented the activity of DnaA protein on DNA topology, binding of the adenine nucleotide may regulate the activity.

UR - http://www.scopus.com/inward/record.url?scp=0029738044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029738044&partnerID=8YFLogxK

U2 - 10.1021/bi953088f

DO - 10.1021/bi953088f

M3 - Article

C2 - 8784207

AN - SCOPUS:0029738044

VL - 35

SP - 11512

EP - 11516

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 35

ER -