Effective cloning of unmarked DNA fragments in the bacillus subtilis 168 genome

Mitsuhiro Itaya

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The Bacillus subtilis 168 genome is being used to clone DNA segments, particularly unmarked DNA segments between the two available clones. To facilitate this cloning, a counter selection method was developed in which loss of a cI repressor gene rendered the host strain resistant to neomycin. This method is promoted to use the B. subtilis genome as a general cloning vehicle.

Original languageEnglish
Pages (from-to)602-604
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume63
Issue number3
DOIs
Publication statusPublished - 1999 Jan 1

Keywords

  • Inducible promoter
  • Positive selection
  • Repressor binding

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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