Effective cloning of unmarked DNA fragments in the Bacillus subtilis 168 genome

Mitsuhiro Itaya

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The Bacillus subtilis 168 genome is being used to clone DNA segments, particularly unmarked DNA segments between the two available clones. To facilitate this cloning, a counter selection method was developed in which loss of a cI repressor gene rendered the host strain resistant to neomycin. This method is promoted to use the B. subtilis genome as a general cloning vehicle.

Original languageEnglish
Pages (from-to)602-604
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume63
Issue number3
Publication statusPublished - 1999 Mar
Externally publishedYes

Fingerprint

Cloning
Bacilli
Bacillus subtilis
Organism Cloning
molecular cloning
Clone Cells
Genes
Genome
clones
host strains
Neomycin
genome
neomycin
DNA
selection methods
genes
methodology

Keywords

  • Inducible promoter
  • Positive selection
  • Repressor binding

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology

Cite this

Effective cloning of unmarked DNA fragments in the Bacillus subtilis 168 genome. / Itaya, Mitsuhiro.

In: Bioscience, Biotechnology and Biochemistry, Vol. 63, No. 3, 03.1999, p. 602-604.

Research output: Contribution to journalArticle

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