Effective cloning of unmarked DNA fragments in the bacillus subtilis 168 genome

Mitsuhiro Itaya

Research output: Contribution to journalArticle

13 Citations (Scopus)


The Bacillus subtilis 168 genome is being used to clone DNA segments, particularly unmarked DNA segments between the two available clones. To facilitate this cloning, a counter selection method was developed in which loss of a cI repressor gene rendered the host strain resistant to neomycin. This method is promoted to use the B. subtilis genome as a general cloning vehicle.

Original languageEnglish
Pages (from-to)602-604
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Issue number3
Publication statusPublished - 1999 Jan 1



  • Inducible promoter
  • Positive selection
  • Repressor binding

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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