Effective expansion of human adipose-derived stromal cells and bone marrow-derived mesenchymal stem cells cultured on a fragmin/protamine nanoparticles-coated substratum with human platelet-rich plasma

Satoko Kishimoto, Masayuki Ishihara, Yasutaka Mori, Megumi Takikawa, Hidemi Hattori, Shingo Nakamura, Toshinori Sato

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Fragmin/protamine nanoparticles (F/P NPs) can be stably coated onto plastic surfaces and used as a substratum for the absorption and controlled release of growth factors (GFs) secreted from human platelet-rich plasma (PRP). In this study, we investigated the capability of F/P NP-coated plates to act as a substratum for the proliferation of human adipose-derived stromal cells (ASCs) and bone marrow-derived mesenchymal stem cells (BMSCs) with GFs in PRP. Both cell types adhered well to the F/P NP-coated plates and grew optimally, with a doubling time of 30 and 32 h in low-concentration PRP (0.5%) medium supplemented with 5 ng/ml fibroblast growth factor-2 (FGF-2) on the F/P NP-coated plates. These cells maintained their multilineage potential for differentiation into adipocytes or osteoblasts. Furthermore, ASCs and BMSCs grew well in medium without PRP and FGF-2 on F/P NP-coated plates pretreated with PRP and FGF-2 in a concentration-dependent manner. Thus, F/P NP-coated plates are a useful substratum for the adherence and proliferation of ASCs and BMSCs in low-concentration PRP medium supplemented with FGF-2. No xenogeneic serum is required.

Original languageEnglish
Pages (from-to)955-964
Number of pages10
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume7
Issue number12
DOIs
Publication statusPublished - 2013 Dec

Fingerprint

Dalteparin
Protamines
Platelet-Rich Plasma
Platelets
Stem cells
Mesenchymal Stromal Cells
Nanoparticles
Bone
Fibroblast Growth Factor 2
Fibroblasts
Plasmas
Intercellular Signaling Peptides and Proteins
Osteoblasts
Adipocytes
Plastics
Serum

Keywords

  • Adipose-derived stromal cells (ASCs)
  • Bone marrow-derived mesenchymal stem cells (BMSCs)
  • Fragmin/protamine nanoparticles (F/P NPs)
  • Growth factors
  • Matrix
  • Platelet-rich plasma (PRP)

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

Effective expansion of human adipose-derived stromal cells and bone marrow-derived mesenchymal stem cells cultured on a fragmin/protamine nanoparticles-coated substratum with human platelet-rich plasma. / Kishimoto, Satoko; Ishihara, Masayuki; Mori, Yasutaka; Takikawa, Megumi; Hattori, Hidemi; Nakamura, Shingo; Sato, Toshinori.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 7, No. 12, 12.2013, p. 955-964.

Research output: Contribution to journalArticle

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