Effective Separation for New Therapeutic Modalities Utilizing Temperature-responsive Chromatography

Yutaro Maekawa, Eri Ayano, Kenichi Nagase, Hideko Kanazawa

Research output: Contribution to journalArticlepeer-review

Abstract

In recent years, drug discovery and therapeutics trends have shifted from a focus on small-molecule compounds to biopharmaceuticals, genes, cell therapy, and regenerative medicine. Therefore, new approaches and technologies must be developed to respond to these changes in medical care. To achieve this, we applied a temperature-responsive separation system to purify a variety of proteins and cells. We developed a temperature-responsive chromatography technique based on a poly(N-isopropylacrylamide) (PNIPAAm)-grafted stationary phase. This method may be applied to various types of protein and cell separation applications by optimizing the properties of the modified polymers used in this system. Therefore, the developed temperature-responsive HPLC columns and temperature-responsive solid-phase extraction (TRSPE) columns can be an effective separation tool for new therapeutic modalities such as monoclonal antibodies, nucleic acid drugs, and cells.

Original languageEnglish
Pages (from-to)651-660
Number of pages10
Journalanalytical sciences
Volume37
Issue number5
DOIs
Publication statusPublished - 2021 May

Keywords

  • Temperature-responsive chromatography
  • cell separation
  • monoclonal antibody
  • new therapeutic modality
  • oligonucleotide
  • poly(N-isopropylacrylamide)
  • smart functional surfaces

ASJC Scopus subject areas

  • Analytical Chemistry

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