Effective separation of peptides using highly dense thermo-responsive polymer brush-grafted porous polystyrene beads

Aya Mizutani, Kenichi Nagase, Akihiko Kikuchi, Hideko Kanazawa, Yoshikatsu Akiyama, Jun Kobayashi, Masahiko Annaka, Teruo Okano

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

For the development of well-defined highly dense thermo-responsive polymer grafted surface as an improved stationary phase for thermo-responsive chromatography, poly(N-isopropylacrylamide) (PIPAAm) brush-grafted porous polystyrene beads were prepared by surface-initiated atom transfer radical polymerization (ATRP). The PIPAAm grafted region of polystyrene beads was adjusted by the addition of isooctane as a poor solvent for polystyrene upon the reaction of ATRP initiator immobilization. Using a thermo-responsive HPLC column containing the prepared beads with PIPAAm brush grafted on the inside pores nearby the outer surfaces, angiotensin subtypes were effectively separated with aqueous mobile phase, because the densely grafted PIPAAm on nearby the outer surface effectively interacted with the peptides hydrophobically. Retention of basic peptide was achieved by the beads with basic mobile phase. These results indicated that the prepared beads with grafted PIPAAm nearby the outer surface became an effective chromatographic stationary phase for retaining basic peptides using wide pH range of mobile phase.

Original languageEnglish
Pages (from-to)2191-2198
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume878
Issue number24
DOIs
Publication statusPublished - 2010 Aug

Fingerprint

Polystyrenes
Brushes
Polymers
Peptides
Atom transfer radical polymerization
Polymerization
Angiotensins
Chromatography
Immobilization
High Pressure Liquid Chromatography
poly-N-isopropylacrylamide

Keywords

  • Atom transfer radical polymerization
  • Peptide
  • Poly(N-isopropylacrylamide)
  • Poly(styrene-co-divinylbenzene)
  • Polymer brush
  • Thermo-responsive chromatography
  • Thermo-responsive polymer

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

Effective separation of peptides using highly dense thermo-responsive polymer brush-grafted porous polystyrene beads. / Mizutani, Aya; Nagase, Kenichi; Kikuchi, Akihiko; Kanazawa, Hideko; Akiyama, Yoshikatsu; Kobayashi, Jun; Annaka, Masahiko; Okano, Teruo.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 878, No. 24, 08.2010, p. 2191-2198.

Research output: Contribution to journalArticle

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AU - Kobayashi, Jun

AU - Annaka, Masahiko

AU - Okano, Teruo

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