Effects of α1-acid glycoprotein on isometric tension of mouse aorta

Yoshiko Tokutomi, Shigehiro Okamoto, Kazuaki Matsumoto, Masaki Otagiri, Katsuhide Nishi, Naofumi Tokutomi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We examined the effects of human α1-acid glycoprotein on isometric tension of mouse aortic rings. α1-Acid glycoprotein (7.5-75 μM) produced a transient, concentration-dependent relaxation of the phenylephrine-precontracted preparation. Although NG-nitro-L- arginine methyl ester or removal of endothelium rarely affected the α1-acid glycoprotein-induced relaxation, extracellular heparin inhibited the α1-acid glycoprotein-induced relaxation. In 10 mM Ca2+-containing external solutions, the α1-acid glycoprotein-induced relaxation was significantly potentiated. In the 60 mM KCl-precontracted preparation, α1-acid glycoprotein produced weaker relaxation than in the phenylephrine-precontracted preparation. These results suggest that the vasorelaxant effect of α1-acid glycoprotein is mainly achieved by block of Ca2+ entry in the vascular smooth muscle cells. The interaction between α1-acid glycoprotein molecules and plasmalemmal Ca2+ entry channels may be modified by extracellular Ca2+ and heparin.

Original languageEnglish
Pages (from-to)137-141
Number of pages5
JournalEuropean journal of pharmacology
Volume477
Issue number2
DOIs
Publication statusPublished - 2003 Sep 12
Externally publishedYes

Keywords

  • Aorta
  • Ca entry
  • Isometric tension
  • Mouse
  • Smooth muscle cell
  • Vascular
  • α-Acid glycoprotein

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Effects of α<sub>1</sub>-acid glycoprotein on isometric tension of mouse aorta'. Together they form a unique fingerprint.

  • Cite this