Effects of 3,3'4)ihydroxy-a,/j-diethylstilbene And 3,3 ',4,5'-tetrahydroxystilbene On Microtubule Assembly In Vitro, Aneuploidy Induction, And Cellular Microtubule And Actin Networks

Yumiko Sakakibara, Izumi Saito, Taiko Oda, Hiroshi Tsujibo, Yoshihiko Inamori, Yoshihiro Sato

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Abstract

We examined the inhibitory activities of 3,3'-dihydroxy-a,/J-diethylstilbene (DDS) and 3,3 ',4,5'-tetrahydroxystilbene (THS) on microtubule assembly in vitro and their effects on chromosome number and cellular microtubule networks in Chinese hamster V79 cells. DDS showed half the inhibitory activity of diethylstilbestrol (DES) on microtubule assembly in vitro, while THS had none of the inhibitory activity. DDS induced tetraploid at 30 /im, whereas THS was found to be inactive. Furthermore, DDS disturbed cellular microtubule networks at 100 pm. We also examined the effects of DES, DDS and THS on cellular actin networks in mouse BALB 3T3 cells. DES induced a change of actin stress fiber distribution and THS had similar activity, while DDS showed no activity.

Original languageEnglish
Pages (from-to)2115-2119
Number of pages5
JournalChemical and Pharmaceutical Bulletin
Volume40
Issue number8
DOIs
Publication statusPublished - 1992 Jan 1

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Keywords

  • 3,3',4,5/-tetrahydroxystilbene
  • 3,3'-dihydroxy-α,β-diethylstilbene
  • Chinese hamster V79 cell
  • actin
  • aneuploidy
  • diethylstilbestrol
  • immunofluorescence
  • microtubule assembly
  • mouse BALB 3T3 cell

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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