Effects of 9-cis retinoic acid on hematopoiesis in vitro

M. Kizaki, H. Nakajima, Y. Ikeda

Research output: Contribution to journalArticle

Abstract

9-cis retinoic acid (cRA) is a high affinity ligand for RXR and binds efficiently to RAR. cRA in general was more potent than all-trans RA (tRA) in suppressing clonal growth and inducing differentiation of HL-60 and human leukemic cells. At very low concentrations, retinoid stimulate proliferation of leukemic cells rather than induce their differentiation. Also, cRA in combination with tRA could induce differentiation of RA resistant HL-60 cells. Steady-state levels of RAR-alpha mRNA in HL-60 cells were not affected by either cRA or tRA. In contrast, cRA, but not tRA could reduced RXR-alpha mRNA accumulation in dose-dependent manner. The loss of partner for RAR may result in differential gene regulation through the retinoid pathway. These differences in regulation of RAR and RXR mRNA might be of physiological significance.

Original languageEnglish
Pages (from-to)256-260
Number of pages5
Journal[Rinsho ketsueki] The Japanese journal of clinical hematology
Volume35
Issue number3
Publication statusPublished - 1994 Mar

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Hematopoiesis
HL-60 Cells
Retinoids
Messenger RNA
Cell Proliferation
In Vitro Techniques
alitretinoin
Ligands
Growth
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effects of 9-cis retinoic acid on hematopoiesis in vitro. / Kizaki, M.; Nakajima, H.; Ikeda, Y.

In: [Rinsho ketsueki] The Japanese journal of clinical hematology, Vol. 35, No. 3, 03.1994, p. 256-260.

Research output: Contribution to journalArticle

Kizaki, M. ; Nakajima, H. ; Ikeda, Y. / Effects of 9-cis retinoic acid on hematopoiesis in vitro. In: [Rinsho ketsueki] The Japanese journal of clinical hematology. 1994 ; Vol. 35, No. 3. pp. 256-260.
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