Abstract
The purpose of this study was to determine the effects of a continuous 80% Percoll density gradient procedure for sperm separation to the ratio of X-bearing sperm and the frequency of disomic sperm for chromosome 18. Semen samples were obtained from normal three donors. Controls and processed sperms (80% percoll fraction) were subjected to fluorescence in situ hybridization (FISH) analysis with DNA probes. FISH was carried out with X-specific DNA probe (DXZ1) and chromosome 18 specific DNA probe (D18Z1). The X-bearing sperm rate of controls ranged from 48.0% to 53.7%. The X-bearing sperm rate of processed spermatozoa ranged from 50.3% to 58.2%. A significantly higher frequency of X-bearing sperm was observed in Percoll washed spermatozoa of one subject (the X-bearing sperm frequency was 48.0: 58.2). No significant difference was found between control sperm and processed sperm for each donor in the distribution of disomy of chromosome 18.
| Original language | English |
|---|---|
| Pages (from-to) | 113-118 |
| Number of pages | 6 |
| Journal | Japanese Journal of Fertility and Sterility |
| Volume | 43 |
| Issue number | 2 |
| Publication status | Published - 1998 |
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Keywords
- Continuous density gradient procedure
- Disomy
- FISH (fluorescence in situ hybridization)
- Percoil
- Sperm
ASJC Scopus subject areas
- Obstetrics and Gynaecology
Cite this
Effects of a continuous percoil gradient procedure for sperm separation. / Asada, H.; Sueoka, Kou; Hashiba, T.; Kuroshima, M.; Tsuchiya, S.; Yoshimura, Y.
In: Japanese Journal of Fertility and Sterility, Vol. 43, No. 2, 1998, p. 113-118.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of a continuous percoil gradient procedure for sperm separation
AU - Asada, H.
AU - Sueoka, Kou
AU - Hashiba, T.
AU - Kuroshima, M.
AU - Tsuchiya, S.
AU - Yoshimura, Y.
PY - 1998
Y1 - 1998
N2 - The purpose of this study was to determine the effects of a continuous 80% Percoll density gradient procedure for sperm separation to the ratio of X-bearing sperm and the frequency of disomic sperm for chromosome 18. Semen samples were obtained from normal three donors. Controls and processed sperms (80% percoll fraction) were subjected to fluorescence in situ hybridization (FISH) analysis with DNA probes. FISH was carried out with X-specific DNA probe (DXZ1) and chromosome 18 specific DNA probe (D18Z1). The X-bearing sperm rate of controls ranged from 48.0% to 53.7%. The X-bearing sperm rate of processed spermatozoa ranged from 50.3% to 58.2%. A significantly higher frequency of X-bearing sperm was observed in Percoll washed spermatozoa of one subject (the X-bearing sperm frequency was 48.0: 58.2). No significant difference was found between control sperm and processed sperm for each donor in the distribution of disomy of chromosome 18.
AB - The purpose of this study was to determine the effects of a continuous 80% Percoll density gradient procedure for sperm separation to the ratio of X-bearing sperm and the frequency of disomic sperm for chromosome 18. Semen samples were obtained from normal three donors. Controls and processed sperms (80% percoll fraction) were subjected to fluorescence in situ hybridization (FISH) analysis with DNA probes. FISH was carried out with X-specific DNA probe (DXZ1) and chromosome 18 specific DNA probe (D18Z1). The X-bearing sperm rate of controls ranged from 48.0% to 53.7%. The X-bearing sperm rate of processed spermatozoa ranged from 50.3% to 58.2%. A significantly higher frequency of X-bearing sperm was observed in Percoll washed spermatozoa of one subject (the X-bearing sperm frequency was 48.0: 58.2). No significant difference was found between control sperm and processed sperm for each donor in the distribution of disomy of chromosome 18.
KW - Continuous density gradient procedure
KW - Disomy
KW - FISH (fluorescence in situ hybridization)
KW - Percoil
KW - Sperm
UR - http://www.scopus.com/inward/record.url?scp=0031750370&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031750370&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0031750370
VL - 43
SP - 113
EP - 118
JO - Japanese Journal of Fertility and Sterility
JF - Japanese Journal of Fertility and Sterility
SN - 0029-0629
IS - 2
ER -