Effects of a continuous percoil gradient procedure for sperm separation

H. Asada, K. Sueoka, T. Hashiba, M. Kuroshima, S. Tsuchiya, Y. Yoshimura

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to determine the effects of a continuous 80% Percoll density gradient procedure for sperm separation to the ratio of X-bearing sperm and the frequency of disomic sperm for chromosome 18. Semen samples were obtained from normal three donors. Controls and processed sperms (80% percoll fraction) were subjected to fluorescence in situ hybridization (FISH) analysis with DNA probes. FISH was carried out with X-specific DNA probe (DXZ1) and chromosome 18 specific DNA probe (D18Z1). The X-bearing sperm rate of controls ranged from 48.0% to 53.7%. The X-bearing sperm rate of processed spermatozoa ranged from 50.3% to 58.2%. A significantly higher frequency of X-bearing sperm was observed in Percoll washed spermatozoa of one subject (the X-bearing sperm frequency was 48.0: 58.2). No significant difference was found between control sperm and processed sperm for each donor in the distribution of disomy of chromosome 18.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalJapanese Journal of Fertility and Sterility
Volume43
Issue number2
Publication statusPublished - 1998 Jan 1

Keywords

  • Continuous density gradient procedure
  • Disomy
  • FISH (fluorescence in situ hybridization)
  • Percoil
  • Sperm

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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    Asada, H., Sueoka, K., Hashiba, T., Kuroshima, M., Tsuchiya, S., & Yoshimura, Y. (1998). Effects of a continuous percoil gradient procedure for sperm separation. Japanese Journal of Fertility and Sterility, 43(2), 113-118.