Effects of a highly basic region of human immunodeficiency virus Tat protein on nucleolar localization

Haruhiko Siomi, H. Shida, M. Maki, M. Hatanaka

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

Human immunodeficiency virus type 1 encodes a positive trans-activator protein, Tat, which is located predominantly in the cell nucleolus. To study the role of the basic region of Tat in nucleolar localization, we constructed fusion genes encoding serially deleted segments of Tat joined to the amino-terminal end of the Escherichia coli β-galactosidase molecule. We show that the basic region of Tat was sufficient for nuclear localization but not for nucleolar localization. Addition of three amino acids (59, 60, and 61) of the Tat sequence at the C-terminal end of the basic region was necessary for the chimeric β-galactosidase to localize in the nucleus as well as in the nucleolus. We demonstrate that a short amino acid sequence (G-48 RKKRRQRRRA HQ N-61), when fused to the amino terminus of β-galactosidase, can act as a nucleolar localization signal.

Original languageEnglish
Pages (from-to)1803-1807
Number of pages5
JournalJournal of Virology
Volume64
Issue number4
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Galactosidases
tat Gene Products
Human Immunodeficiency Virus Proteins
galactosidases
Human immunodeficiency virus
cell nucleolus
Cell Nucleolus
Trans-Activators
gene fusion
proteins
Gene Fusion
Human immunodeficiency virus 1
HIV-1
Amino Acid Sequence
amino acid sequences
Escherichia coli
Amino Acids
amino acids

ASJC Scopus subject areas

  • Immunology

Cite this

Effects of a highly basic region of human immunodeficiency virus Tat protein on nucleolar localization. / Siomi, Haruhiko; Shida, H.; Maki, M.; Hatanaka, M.

In: Journal of Virology, Vol. 64, No. 4, 1990, p. 1803-1807.

Research output: Contribution to journalArticle

@article{e9bab96733ae4430b86398d81c8d03b1,
title = "Effects of a highly basic region of human immunodeficiency virus Tat protein on nucleolar localization",
abstract = "Human immunodeficiency virus type 1 encodes a positive trans-activator protein, Tat, which is located predominantly in the cell nucleolus. To study the role of the basic region of Tat in nucleolar localization, we constructed fusion genes encoding serially deleted segments of Tat joined to the amino-terminal end of the Escherichia coli β-galactosidase molecule. We show that the basic region of Tat was sufficient for nuclear localization but not for nucleolar localization. Addition of three amino acids (59, 60, and 61) of the Tat sequence at the C-terminal end of the basic region was necessary for the chimeric β-galactosidase to localize in the nucleus as well as in the nucleolus. We demonstrate that a short amino acid sequence (G-48 RKKRRQRRRA HQ N-61), when fused to the amino terminus of β-galactosidase, can act as a nucleolar localization signal.",
author = "Haruhiko Siomi and H. Shida and M. Maki and M. Hatanaka",
year = "1990",
language = "English",
volume = "64",
pages = "1803--1807",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "4",

}

TY - JOUR

T1 - Effects of a highly basic region of human immunodeficiency virus Tat protein on nucleolar localization

AU - Siomi, Haruhiko

AU - Shida, H.

AU - Maki, M.

AU - Hatanaka, M.

PY - 1990

Y1 - 1990

N2 - Human immunodeficiency virus type 1 encodes a positive trans-activator protein, Tat, which is located predominantly in the cell nucleolus. To study the role of the basic region of Tat in nucleolar localization, we constructed fusion genes encoding serially deleted segments of Tat joined to the amino-terminal end of the Escherichia coli β-galactosidase molecule. We show that the basic region of Tat was sufficient for nuclear localization but not for nucleolar localization. Addition of three amino acids (59, 60, and 61) of the Tat sequence at the C-terminal end of the basic region was necessary for the chimeric β-galactosidase to localize in the nucleus as well as in the nucleolus. We demonstrate that a short amino acid sequence (G-48 RKKRRQRRRA HQ N-61), when fused to the amino terminus of β-galactosidase, can act as a nucleolar localization signal.

AB - Human immunodeficiency virus type 1 encodes a positive trans-activator protein, Tat, which is located predominantly in the cell nucleolus. To study the role of the basic region of Tat in nucleolar localization, we constructed fusion genes encoding serially deleted segments of Tat joined to the amino-terminal end of the Escherichia coli β-galactosidase molecule. We show that the basic region of Tat was sufficient for nuclear localization but not for nucleolar localization. Addition of three amino acids (59, 60, and 61) of the Tat sequence at the C-terminal end of the basic region was necessary for the chimeric β-galactosidase to localize in the nucleus as well as in the nucleolus. We demonstrate that a short amino acid sequence (G-48 RKKRRQRRRA HQ N-61), when fused to the amino terminus of β-galactosidase, can act as a nucleolar localization signal.

UR - http://www.scopus.com/inward/record.url?scp=0025239390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025239390&partnerID=8YFLogxK

M3 - Article

C2 - 2108259

AN - SCOPUS:0025239390

VL - 64

SP - 1803

EP - 1807

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 4

ER -