Effects of adenine-nucleotides on the sequence-specificity of origin recognition complex-binding to DNA

Hitomi Takenaka, Naoko Takahashi, Jong Ryul Lee, Masaki Makise, Yoshihiro Yamaguchi, Tomofusa Tsuchiya, Tohru Mizushima

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We examined effects of adenine-nucleotides on the sequence-specificity of origin recognition complex (ORC)-binding to DNA, using a gel electrophoretic mobility shift assay. The sequence-specific DNA binding of ORC was observed in the presence of ATP or ATP-γ-S but not in the presence of adenosine 5′-diphosphate (ADP) or in the absence of any adenine-nucleotides. In contrast, the sequence-independent DNA binding of ORC was observed under any one of these conditions. These results suggest that ATP increases the sequence-specificity of ORC-binding to DNA. In relation to the requirement for incubation at high temperature and inhibition by cardiolipin, there was no significant difference between the sequence-specific and the sequence-independent DNA binding activities of ORC.

Original languageEnglish
Pages (from-to)652-655
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume25
Issue number5
DOIs
Publication statusPublished - 2002 May
Externally publishedYes

Fingerprint

Origin Recognition Complex
Adenine Nucleotides
DNA
Adenosine Triphosphate
Cardiolipins
Electrophoretic Mobility Shift Assay
Adenosine Diphosphate
Gels
Temperature

Keywords

  • Adenine-nucleotide
  • DNA replication
  • ORC
  • Origin DNA

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Takenaka, H., Takahashi, N., Lee, J. R., Makise, M., Yamaguchi, Y., Tsuchiya, T., & Mizushima, T. (2002). Effects of adenine-nucleotides on the sequence-specificity of origin recognition complex-binding to DNA. Biological and Pharmaceutical Bulletin, 25(5), 652-655. https://doi.org/10.1248/bpb.25.652

Effects of adenine-nucleotides on the sequence-specificity of origin recognition complex-binding to DNA. / Takenaka, Hitomi; Takahashi, Naoko; Lee, Jong Ryul; Makise, Masaki; Yamaguchi, Yoshihiro; Tsuchiya, Tomofusa; Mizushima, Tohru.

In: Biological and Pharmaceutical Bulletin, Vol. 25, No. 5, 05.2002, p. 652-655.

Research output: Contribution to journalArticle

Takenaka, H, Takahashi, N, Lee, JR, Makise, M, Yamaguchi, Y, Tsuchiya, T & Mizushima, T 2002, 'Effects of adenine-nucleotides on the sequence-specificity of origin recognition complex-binding to DNA', Biological and Pharmaceutical Bulletin, vol. 25, no. 5, pp. 652-655. https://doi.org/10.1248/bpb.25.652
Takenaka, Hitomi ; Takahashi, Naoko ; Lee, Jong Ryul ; Makise, Masaki ; Yamaguchi, Yoshihiro ; Tsuchiya, Tomofusa ; Mizushima, Tohru. / Effects of adenine-nucleotides on the sequence-specificity of origin recognition complex-binding to DNA. In: Biological and Pharmaceutical Bulletin. 2002 ; Vol. 25, No. 5. pp. 652-655.
@article{a04460f4d4a04b23947a4c34d8446074,
title = "Effects of adenine-nucleotides on the sequence-specificity of origin recognition complex-binding to DNA",
abstract = "We examined effects of adenine-nucleotides on the sequence-specificity of origin recognition complex (ORC)-binding to DNA, using a gel electrophoretic mobility shift assay. The sequence-specific DNA binding of ORC was observed in the presence of ATP or ATP-γ-S but not in the presence of adenosine 5′-diphosphate (ADP) or in the absence of any adenine-nucleotides. In contrast, the sequence-independent DNA binding of ORC was observed under any one of these conditions. These results suggest that ATP increases the sequence-specificity of ORC-binding to DNA. In relation to the requirement for incubation at high temperature and inhibition by cardiolipin, there was no significant difference between the sequence-specific and the sequence-independent DNA binding activities of ORC.",
keywords = "Adenine-nucleotide, DNA replication, ORC, Origin DNA",
author = "Hitomi Takenaka and Naoko Takahashi and Lee, {Jong Ryul} and Masaki Makise and Yoshihiro Yamaguchi and Tomofusa Tsuchiya and Tohru Mizushima",
year = "2002",
month = "5",
doi = "10.1248/bpb.25.652",
language = "English",
volume = "25",
pages = "652--655",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "5",

}

TY - JOUR

T1 - Effects of adenine-nucleotides on the sequence-specificity of origin recognition complex-binding to DNA

AU - Takenaka, Hitomi

AU - Takahashi, Naoko

AU - Lee, Jong Ryul

AU - Makise, Masaki

AU - Yamaguchi, Yoshihiro

AU - Tsuchiya, Tomofusa

AU - Mizushima, Tohru

PY - 2002/5

Y1 - 2002/5

N2 - We examined effects of adenine-nucleotides on the sequence-specificity of origin recognition complex (ORC)-binding to DNA, using a gel electrophoretic mobility shift assay. The sequence-specific DNA binding of ORC was observed in the presence of ATP or ATP-γ-S but not in the presence of adenosine 5′-diphosphate (ADP) or in the absence of any adenine-nucleotides. In contrast, the sequence-independent DNA binding of ORC was observed under any one of these conditions. These results suggest that ATP increases the sequence-specificity of ORC-binding to DNA. In relation to the requirement for incubation at high temperature and inhibition by cardiolipin, there was no significant difference between the sequence-specific and the sequence-independent DNA binding activities of ORC.

AB - We examined effects of adenine-nucleotides on the sequence-specificity of origin recognition complex (ORC)-binding to DNA, using a gel electrophoretic mobility shift assay. The sequence-specific DNA binding of ORC was observed in the presence of ATP or ATP-γ-S but not in the presence of adenosine 5′-diphosphate (ADP) or in the absence of any adenine-nucleotides. In contrast, the sequence-independent DNA binding of ORC was observed under any one of these conditions. These results suggest that ATP increases the sequence-specificity of ORC-binding to DNA. In relation to the requirement for incubation at high temperature and inhibition by cardiolipin, there was no significant difference between the sequence-specific and the sequence-independent DNA binding activities of ORC.

KW - Adenine-nucleotide

KW - DNA replication

KW - ORC

KW - Origin DNA

UR - http://www.scopus.com/inward/record.url?scp=0036581972&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036581972&partnerID=8YFLogxK

U2 - 10.1248/bpb.25.652

DO - 10.1248/bpb.25.652

M3 - Article

C2 - 12033508

AN - SCOPUS:0036581972

VL - 25

SP - 652

EP - 655

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 5

ER -