Effects of adenine-nucleotides on the sequence-specificity of origin recognition complex-binding to DNA

Hitomi Takenaka, Naoko Takahashi, Jong Ryul Lee, Masaki Makise, Yoshihiro Yamaguchi, Tomofusa Tsuchiya, Tohru Mizushima

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We examined effects of adenine-nucleotides on the sequence-specificity of origin recognition complex (ORC)-binding to DNA, using a gel electrophoretic mobility shift assay. The sequence-specific DNA binding of ORC was observed in the presence of ATP or ATP-γ-S but not in the presence of adenosine 5′-diphosphate (ADP) or in the absence of any adenine-nucleotides. In contrast, the sequence-independent DNA binding of ORC was observed under any one of these conditions. These results suggest that ATP increases the sequence-specificity of ORC-binding to DNA. In relation to the requirement for incubation at high temperature and inhibition by cardiolipin, there was no significant difference between the sequence-specific and the sequence-independent DNA binding activities of ORC.

Original languageEnglish
Pages (from-to)652-655
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume25
Issue number5
DOIs
Publication statusPublished - 2002 May 1

Keywords

  • Adenine-nucleotide
  • DNA replication
  • ORC
  • Origin DNA

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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  • Cite this

    Takenaka, H., Takahashi, N., Lee, J. R., Makise, M., Yamaguchi, Y., Tsuchiya, T., & Mizushima, T. (2002). Effects of adenine-nucleotides on the sequence-specificity of origin recognition complex-binding to DNA. Biological and Pharmaceutical Bulletin, 25(5), 652-655. https://doi.org/10.1248/bpb.25.652